Molecular analysis of flies selected for aggressive behavior
Molecular analysis of flies selected for aggressive behavior
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DOI：
10.1038/ng1864
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发表时间：
2006-09-01
期刊：
影响因子：
30.8
通讯作者：
Greenspan, Ralph J.
中科院分区：
文献类型：
--
作者：
Dierick, Herman A.;Greenspan, Ralph J.
攻击性行为在整个动物王国中普遍存在，但人们对其分子基础知之甚少。为了解决这个问题，我们开发了一种基于种群的选择程序，以增加果蝇的攻击性。我们用一种新的双雄竞技场试验测量了所选亚群中攻击行为的变化。在仅仅10代的选择中，攻击性品系变得明显比中性品系更具攻击性。21代后，战斗指数增加了30倍以上。使用微阵列分析，我们确定了具有不同表达水平的基因在侵略性和中性线作为候选人，这种强烈的行为选择反应。我们通过突变分析测试了一小部分基因，发现其中一个基因显著增加了战斗频率。这些结果表明，增加攻击性的选择可以用来从分子上剖析这种行为。
Aggressive behavior is pervasive throughout the animal kingdom, and yet very little is known about its molecular underpinnings. To address this problem, we have developed a population-based selection procedure to increase aggression in Drosophila melanogaster. We measured changes in aggressive behavior in the selected subpopulations with a new two-male arena assay. In only ten generations of selection, the aggressive lines became markedly more aggressive than the neutral lines. After 21 generations, the fighting index increased more than 30-fold. Using microarray analysis, we identified genes with differing expression levels in the aggressive and neutral lines as candidates for this strong behavioral selection response. We tested a small set of these genes through mutant analysis and found that one significantly increased fighting frequency. These results suggest that selection for increases in aggression can be used to molecularly dissect this behavior.