Development of VNTR markers for two Fusarium graminearum clade species

Development of VNTR markers for two Fusarium graminearum clade species
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DOI:
10.1111/j.1471-8286.2004.00703.x
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发表时间:
2004-09
期刊:
Molecular Ecology Notes
影响因子:
--
通讯作者:
H. Suga;L. Gale;H. Kistler
H. Suga;L. Gale;H. Kistler
中科院分区:
其他
文献类型:
--
作者:
H. Suga;L. Gale;H. Kistler

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利用生物信息学的方法,我们开发了10个可变数目的串联重复序列(VNTR)标记的禾谷镰刀菌和亚洲镰刀菌有用的群体遗传研究。在F.通过串联重复序列发现程序鉴定禾谷镰刀菌。对来自美国、意大利和中国的5个菌株进行了54个位点的长度多态性检测。从这54个基因座中,选择了10个基于跨物种检测到的多态性,易于评分,以及它们在基因组中的分散位置。
Using a bioinformatics approach, we developed 10 variable number of tandem repeat (VNTR) markers for Fusarium graminearum and Fusarium asiaticum useful for population genetic studies. Repeat sequences in the genome sequence of F. graminearum were identified by a tandem repeat finding program. Length polymorphisms at 54 loci were examined for five strains each from the United States, Italy and China. From these 54 loci, 10 were selected based on polymorphisms detected across species, ease of scoring, and their dispersed location in the genome.