The Soybean Gene J Contributes to Salt Stress Tolerance by Up-Regulating Salt-Responsive Genes
The Soybean Gene J Contributes to Salt Stress Tolerance by Up-Regulating Salt-Responsive Genes
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大豆基因 J 通过上调盐响应基因来增强盐胁迫耐受性
DOI：
10.3389/fpls.2020.00272
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发表时间：
2020-03-17
影响因子：
5.6
通讯作者：
Dong, Lidong
中科院分区：
文献类型：
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作者：
Cheng, Qun;Gan, Zhuoran;Dong, Lidong
大豆[Glycine max（L.）Merr.]是世界范围内重要的石油和蛋白质资源作物，其种植受到土壤盐分水平上升的影响。在拟南芥中，基因EARLY FLOWARD 3（ELF 3）通过抑制盐胁迫响应途径来增加耐盐性。J是AtELF 3在大豆中的直向同源基因，功能缺失的J等位基因可显著延长大豆成熟期，提高籽粒产量。然而，J在大豆非生物胁迫反应中的确切作用仍不清楚。在这项研究中，我们表明，J的表达诱导NaCl处理和J蛋白位于细胞核中。与NIL-J相比，NIL-j突变体对NaCl的耐受性显著降低。我们还证明了J的过表达增加了转基因大豆毛状根的耐盐性。J正调控下游盐胁迫响应基因的表达，包括GmWRKY 12、GmWRKY 27、GmWRKY 54、GmNAC和GmSIN 1。我们的研究揭示了大豆盐胁迫反应的调控机制。这些基因的操作将有助于提高大豆的耐盐性。
Soybean [Glycine max (L.) Merr.] is an important crop for oil and protein resources worldwide, and its farming is impacted by increasing soil salinity levels. In Arabidopsis the gene EARLY FLOWERING 3 (ELF3), increased salt tolerance by suppressing salt stress response pathways. J is the ortholog of AtELF3 in soybean, and loss-of-function J-alleles greatly prolong soybean maturity and enhance grain yield. The exact role of J in abiotic stress response in soybean, however, remains unclear. In this study, we showed that J expression was induced by NaCl treatment and that the J protein was located in the nucleus. Compared to NIL-J, tolerance to NaCl was significantly lower in the NIL-j mutant. We also demonstrated that overexpression of J increased NaCl tolerance in transgenic soybean hairy roots. J positively regulated expression of downstream salt stress response genes, including GmWRKY12, GmWRKY27, GmWRKY54, GmNAC, and GmSIN1. Our study disclosed a mechanism in soybean for regulation of the salt stress response. Manipulation of these genes should facilitate improvements in salt tolerance in soybean.