Segregation of Microsatellite Alleles in Gynogenetic Diploid Pacific Abalone (Haliotis discus hannai)
Segregation of Microsatellite Alleles in Gynogenetic Diploid Pacific Abalone (Haliotis discus hannai)
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DOI：
10.1007/s10126-004-5119-1
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发表时间：
2005-08
影响因子：
3
通讯作者：
Qi Li;A. Kijima
中科院分区：
文献类型：
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作者：
Qi Li;A. Kijima
用紫外线照射的精子抑制皱纹盘鲍的第二次减数分裂，并将其受精卵与第二次减数分裂抑制后，对皱纹盘鲍雌核发育的3个家系进行了9个微卫星位点的遗传分析。杂合后代的比例用于估计标记-着丝粒（M-C）距离。当无效等位基因被占时，所有位点在对照杂交中符合孟德尔分离。父本等位基因的缺失证实了后代的雌核发育起源，并表明3个家庭的成功率为100%。估计的重组频率范围为0.10至0.60，低于在其他雌核发育二倍体动物中观察到的频率。平均重组频率为0.22，对应的固定指数为0.78在一个世代。这是3.12倍的纯合性增加后，预期一个世代的同胞交配（0.25），这表明减数分裂雌核发育可能是一种有效的手段，快速近亲繁殖的鲍鱼。在完全干扰的假设下，9个位点的M-C图距在5 - 30 cM之间变化。M-C距离的信息将有助于进一步的基因定位。汉内铁饼。
Inheritance of 9 microsatellite loci was examined in 3 families of gynogenetic Pacific abaloneHaliotis discus hannaiproduced by fertilizing eggs with UV-irradiated sperm followed by inhibition of the second meiotic division. The proportion of heterozygous progeny was used to estimate marker-centromere (M-C) distances. All loci conformed to Mendelian segregation in the control crosses when null alleles were accounted for. The absence of paternal alleles confirmed the gynogenetic origin of the offspring and indicated 100% success for 3 families. Estimated recombinant frequencies ranged from 0.10 to 0.60, which is lower than those observed in other gynogenetic diploid animals. The mean recombination frequency was 0.22, corresponding to a fixation index of 0.78 in one generation. This is 3.12 times the increase in homozygosity expected after one generation of sib mating (0.25), suggesting meiotic gynogenesis may be an effective means of rapid inbreeding in the abalone. M-C map distances for the 9 loci varied between 5 and 30 cM under the assumption of complete interference. The information about M-C distances will be useful for future gene mapping inH. discus hannai.