Adipose depot-specific effects of ileal interposition surgery in UCD-T2D rats: unexpected implications for obesity and diabetes.
Adipose depot-specific effects of ileal interposition surgery in UCD-T2D rats: unexpected implications for obesity and diabetes.
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DOI：
10.1042/bcj20170899
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发表时间：
2018-02-14
期刊：
影响因子：
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通讯作者：
Giulivi C
中科院分区：
文献类型：
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作者：
Hung C;Bronec C;Napoli E;Graham J;Stanhope KL;Marsilio I;Giron MC;Havel PJ;Giulivi C
回肠间置（IT）手术延迟了2型糖尿病大鼠模型（UCD-T2 DM）的糖尿病发作。在此，为了更深入地了解IT手术影响的分子事件，我们检查了IT或假手术后1.5个月糖尿病前期大鼠4个白色脂肪库（腹膜后、肠系膜、腹股沟和附睾）的蛋白质组和血浆游离脂肪酸谱的变化。IT介导的变化主要发生在肠系膜脂肪，从延迟脂肪细胞成熟到神经内分泌重塑。相反，腹股沟、腹膜后和附睾贮库显示出相反的趋势，这与糖尿病明显体征之前脂肪细胞成熟和脂肪生成发展增加一致，可能是由PPARγ信号传导和较高的血浆n-6/n-3 FFA比值引起的。由此产生的情况表明，有针对性地使用手术策略，寻求延迟或改善糖尿病，以操纵脂肪库特异性反应，以最大限度地延长手术的持续时间和有益效果。
Ileal interposition (IT) surgery delays the onset of diabetes in a rat model of type-2 diabetes (UCD-T2DM). Here, to gain a deeper understanding of the molecular events underlying the effects of IT surgery, we examined the changes in the proteome of 4 white adipose depots (retroperitoneal, mesenteric, inguinal, and epididymal), and plasma free fatty acid profile in pre-diabetic rats 1.5 months following IT or sham surgery. The IT-mediated changes were exerted mainly in mesenteric fat, and spanned from delayed adipocyte maturation to a neuroendocrine remodeling. Conversely, inguinal, retroperitoneal and epididymal depots showed opposite trends consistent with increased adipocyte maturation and adipogenesis development prior overt signs of diabetes, likely orchestrated by PPARγ signaling and higher plasma n-6/n-3 FFA ratios. The resulting scenario suggests a targeted use of surgical strategies that seek to delay or improve diabetes in order to manipulate adipose depot-specific responses to maximize the duration and beneficial effects of the surgery.