Studies of cerebrospinal fluid flow and penetration into brain following lateral ventricle and cisterna magna injections of the tracer [14C]inulin in rat.
Studies of cerebrospinal fluid flow and penetration into brain following lateral ventricle and cisterna magna injections of the tracer [14C]inulin in rat.
复制标题
对大鼠侧脑室和小脑延髓池注射示踪剂 [14C]菊糖后脑脊液流动和渗入大脑的研究。
DOI：
10.1016/s0306-4522(99)00417-0
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Herkenham,M
中科院分区：
文献类型：
--
作者：
Proescholdt,MG;Hutto,B;Brady,LS;Herkenham,M
突触旁通讯，也称为容量传输，被认为是介导中枢神经系统内信息传递的重要手段。本研究的目的是通过放射自显影术将惰性细胞外标记物[14C]菊粉注射到侧脑室或小脑延髓池后，观察细胞外空间内液体运动的可用通道。通过长期植入的插管对清醒的大鼠进行5μl 1μCi的[14C]菊糖的推注。存活时间为 5 分钟至 4 小时后，对大脑进行处理以进行体内放射自显影。 5 分钟时，示踪剂分布在注射部位“下游”的整个心室、蛛网膜下腔和水池。从这些部位渗透到大脑是复杂的，优先沿着大脑腹侧进入，特别是进入下丘脑和脑干。到 4 小时时，几乎整个大脑都被标记，无论示踪剂应用的位置如何。示踪剂从蛛网膜下腔持续进入表明某些区域充当了捕获循环物质的仓库。这种机制可能有助于在以后的时间点形成深度渗透的模式。整个大脑中流体运动的空间和时间特征对于解释许多涉及将分子注射到脑脊液中的实验程序具有指导意义。
Parasynaptic communication, also termed volume transmission, has been suggested as an important means to mediate information transfer within the central nervous system. The purpose of the present study was to visualize by autoradiography the available channels for fluid movement within the extracellular space following injection of the inert extracellular marker [14C]inulin into the lateral ventricle or cisterna magna. Bolus injections of 5μl of 1μCi of [14C]inulin were made in awake rats via chronically implanted cannulae. After survival times ranging from 5min to 4h, brains were processed for in vivo autoradiography. At 5min the tracer distributed throughout the ventricles, subarachnoid spaces and cisterns “downstream” of the injection sites. Penetration into the brain from these sites was complex with preferential entry along the ventral side of the brain, especially into the hypothalamus and brainstem. By 4h virtually the entire brain was labeled irrespective of the site of tracer application. Sustained tracer entry from subarachnoid spaces suggests that some areas act as depots to trap circulating material. This mechanism may contribute to the pattern of deep penetration at later time-points. The spatial and temporal characteristics of fluid movement throughout the brain are instructive in the interpretation of many experimental procedures involving injection of molecules into the cerebrospinal fluid.