Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs
Introgression between wild and cultivated soybeans of Japan revealed by RFLP analysis for chloroplast DNAs
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DOI：
10.1007/bf02866640
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发表时间：
1999-07-01
期刊：
影响因子：
2.6
通讯作者：
Shimamoto, Y
中科院分区：
文献类型：
--
作者：
Abe, J;Hasegawa, A;Shimamoto, Y
对日本采集的野生大豆进行了叶绿体 DNA RFLP 的调查。在带有 cpDNA 克隆的 RFLP 中检测到了三种单倍型，该克隆包含与 IR 左侧成员相邻的 LSC 区域。大多数测试的植物具有单倍型 III，少数植物（主要收集于日本南部）具有单倍型 II。单倍型 I 是现代品种的主要形式，在四个相距较远的区域前面的六个地点检测到。我们的结果表明，单倍型 III 在日本野生大豆中占主导地位。一些具有单倍型 I 的植物在表型上介于野生大豆和栽培大豆之间，而其他植物则具有普通野生大豆典型的种子形态和植物结构。具有单倍型I的植物似乎是野生大豆和栽培大豆杂交的衍生物，或者是具有单倍型I叶绿体基因组的大豆栽培品种的直系祖先的遗迹。
Wild soybeans collected in Japan were surveyed for RFLPs of chloroplast DNA. Three haplotypes were detected in RFLPs with a cpDNA clone which contains a LSC region adjacent to the left member of IR. Most of the plants tested possessed haplotype III, and a few plants, collected mostly in southern Japan, had haplotype II. Haplotype I, which is the predominant form in modern cultivars, was detected at six sites front four widely separated regions. Our results indicate that haplotype III is predominant in wild soybean of Japan. Some of the plants having haplotype I were phenotypically intermediate between wild and cultivated soybeans, while the others possessed a seed morphology and plant architecture typical of ordinary wild soybean. The plants having haplotype I appear to be either derivatives of hybridization between wild and cultivated soybeans or relics of a direct progenitor of soybean cultivars with the haplotype I chloroplast genome.