Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid, salicylic acid, and ethylene signaling
Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid, salicylic acid, and ethylene signaling
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DOI：
10.1094/mpmi-20-11-1384
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Stotz, Henrik U.
中科院分区：
文献类型：
--
作者：
Guo, Xiaomei;Stotz, Henrik U.
拟南芥对核盘菌的敏感性基因型差异尚未报道，由于这种十字花科植物的极端敏感性。为了克服这一限制，我们建立了接种条件，使敏感性的差异，以S的评价。拟南芥突变体和生态型之间的sclerotiorum。两个coil突变等位基因赋予了对S.菌核用S.菌核。coil-2突变体对S.菌核病与草酸敏感性无关。突变体npr 1和ein 2对S.菌核在ein 2和npr 1突变体中，PDF1.2和发病相关基因PR 1的诱导分别减少。Actigard是一种全身获得性耐药诱导剂苯并噻二唑的商业制剂，可降低对沙门氏菌的敏感性。菌核通过对S.草酸盐引起过氧化氢产生减少，但在植物超氧化物产生或基因表达中没有可检测的变化。
Genotypic differences in susceptibility of Arabidopsis thaliana to Sclerotinia sclerotiorum have not been reported due to the extreme susceptibility of this cruciferous plant. To overcome this limitation, we have established inoculation conditions that enable evaluation of differences in susceptibility to S. sclerotiorum among Arabidopsis mutants and ecotypes. Two coil mutant alleles conferred hypersusceptibility to S. sclerotiorum. The plant defensin gene PDF1.2 was no longer induced after challenging the coil-2 mutant with S. sclerotiorum. Hypersusceptibility of the coil-2 mutant to S. sclerotiorum was not correlated with oxalate sensitivity. The mutants npr1 and ein2 were also hypersusceptible to S. sclerotiorum. Induction of PDF1.2 and the pathogenesis-related gene PR1 was reduced in ein2 and npr1 mutants, respectively. Actigard, a commercial formulation of the systemic acquired resistance inducer benzothiadiazole, reduced susceptibility to S. sclerotiorum. Based on histochemical analysis of oxalate-deficient and wild-type strains of S. sclerotiorum, oxalate caused a decrease in hydrogen peroxide production but no detectable changes in plant superoxide production or gene expression.