Effect of microgravity on the biomechanical properties of lumbar and caudal intervertebral discs in mice
Effect of microgravity on the biomechanical properties of lumbar and caudal intervertebral discs in mice
复制标题
DOI：
10.1016/j.jbiomech.2014.07.005
复制
发表时间：
2014-09-22
影响因子：
2.4
通讯作者：
Lotz, Jeffrey C.
中科院分区：
文献类型：
--
作者：
Bailey, Jeannie F.;Hargens, Alan R.;Lotz, Jeffrey C.
长期暴露于微重力环境已显示对人体脊柱有有害影响，表现为航天飞行期间腰痛和航天飞行后髓核突出的发生率增加。我们研究了微重力对执行了15天STS-131航天飞行任务的小鼠腰椎间盘和尾椎间盘生物力学特性的影响。16只C57BL/C小鼠（航天组，n=8；地面对照组，n=8）在航天后立即处死。原位测量生理盘高度（PDH），并进行压缩蠕变试验，将生物力学特性参数化为终板渗透率(k)、核膨胀压力应变依赖性(D)和环空粘弹性(G)。对于尾盘，与对照组相比，航天组的PDH降低32%，D降低70%，爬行更多（p=0.03）。对于腰椎间盘，各组间PDH和D均无显著差异。腰椎间盘和尾椎间盘的初始模量、渗透压、k和G不受微重力的影响（p < 0.05）。PDH和D的降低表明长时间微重力有效地降低了尾椎间盘的生物力学特性。相比之下，腰椎间盘没有发现差异。这种长期失重和脊柱运动范围差异之间潜在的有害相互作用可能是在宇航员中观察到的颈椎间盘和腰椎间盘突出率增加的基础。(C) 2014年Elsevier Ltd.出版
Prolonged exposure to microgravity has shown to have deleterious effects on the human spine, indicated by low back pain during spaceflight and increased incidence of post-spaceflight herniated nucleus pulposus. We examined the effect of microgravity on biomechanical properties of lumbar and caudal discs from mice having been on 15-day shuttle mission STS-131. Sixteen C57BL/C mice (spaceflight group, n=8; ground-based control group, n=8) were sacrificed immediately after spaceflight. Physiological disc height (PDH) was measured in situ, and compressive creep tests were performed to parameterize biomechanical properties into endplate permeability (k), nuclear swelling pressure strain dependence (D), and annular viscoelasticity (G). For caudal discs, the spaceflight group exhibited 32% lower PDH, 70% lower D and crept more compared to the control mice (p=0.03). For lumbar discs, neither PDH nor D was significantly different between murine groups. Initial modulus, osmotic pressure, k and G for lumbar and caudal discs did not appear influenced by microgravity (p > 0.05). Decreases in both PDH and D suggest prolonged microgravity effectively diminished biomechanical properties of caudal discs. By contrast, differences were not noted for lumbar discs. This potentially deleterious interaction between prolonged weightlessness and differential ranges of motion along the spine may underlie the increased cervical versus lumbar disc herniation rates observed among astronauts. (C) 2014 Published by Elsevier Ltd.