Identification of a microRNA regulator for axon guidance in the olfactory bulb of adult mice
Identification of a microRNA regulator for axon guidance in the olfactory bulb of adult mice
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成年小鼠嗅球轴突引导的 microRNA 调节因子的鉴定
DOI：
10.1016/j.gene.2014.06.063
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发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
Ling Shucai
中科院分区：
文献类型：
--
作者：
Sun Tingting;Li Shanshan;Yang Jing;Yin Yifei;Ling Shucai
Semaphorin 3A（sema 3a）主要定位于嗅神经元层和球周层，通过其动态时空表达对胚胎和成年小鼠嗅球中轴突的正常排列起重要作用。然而，能够通过直接相互作用来调节sema 3a表达的调节剂是未知的。为了寻找嗅球中sema 3a的调控因子，我们将重点放在microRNA上，这是众所周知的转录后调控因子。我们通过定制的microRNA阵列、基因本体论和京都基因百科全书注释，筛选出成熟小鼠嗅球中丰富的microRNA并对其功能进行了研究，发现轴突导向是成年小鼠稳态嗅球中进行的主要分子生物学过程。此外，我们还通过实时定量聚合酶链反应和免疫组织化学追踪了三种候选调控因子（miR-30 c，miR-200 b和miR-429）和sema 3a的表达。结果显示，只有miR-30 c的表达与sema 3a呈负相关。最后，通过双荧光素酶报告基因体外检测，证实miR-30 c是sema 3a的特异性调控因子。综上所述，我们的研究结果表明miR-30 c可能通过抑制sema 3a的表达而参与轴突导向，这将为阐明嗅球的结构和功能维持机制提供新的见解。
Semaphorin3A (sema3a), mainly localized in the olfactory neuron layer and periglomerular layer, is essential for the normal arrangement of axons in the olfactory bulb both in embryonic and adult mice functioning through its dynamic spatiotemporal expression. The regulators that can modulate the expression of sema3a by direct interaction, however, are unknown. In order to find the regulators of sema3a in the olfactory bulb, we focused on microRNAs, well-known post-transcriptional regulators. We found that axon guidance is the main molecular and biological process ongoing in the steady-state olfactory bulb of the adult mouse by screening the abundant microRNAs and exploring their functions in the olfactory bulbviaour customized microRNA arrays, Gene Ontology and Kyoto Encyclopedia of Genes annotation. Furthermore, we traced the expression of three candidate regulators (miR-30c, miR-200b, and miR-429) and sema3a by the quantitative real-time polymerase chain reaction and immunohistochemistry. The results showed that only miR-30c expression corresponded inversely with sema3a. Finally, miR-30c was verified to be a specific regulator of sema3a by dual luciferase reporter assayin vitro. Taken together, our results suggested that miR-30c is a potential regulator in axon-guidance by suppressing the expression of sema3a, which will give new insights in elucidating the mechanism of architectonic and functional maintenance of the olfactory bulb.