Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds
Extension of oil biosynthesis during the mid-phase of seed development enhances oil content in Arabidopsis seeds
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DOI：
10.1111/pbi.12489
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发表时间：
2016-05-01
影响因子：
13.8
通讯作者：
Nishimura, Mikio
中科院分区：
文献类型：
--
作者：
Kanai, Masatake;Mano, Shoji;Nishimura, Mikio
植物种子中油生物合成的调节已被广泛研究，并且已设计生物技术方法来增加种子油含量。在种子中，油和蛋白质的合成呈负相关，但控制这两种途径之间相互作用的机制尚不清楚。在这里，我们确定控制种子中的油和蛋白质含量的分子机制。我们利用转基因拟南芥植物过表达WRINKLED 1（WRI 1），一个主转录因子调节种子油的生物合成，和敲除突变体的主要种子储存蛋白。在野生型植物中，油和蛋白质的生物合成分别在种子发育的早期和晚期被依次激活。种子中的油和蛋白质含量之间的负相关性来自于途径之间的竞争。在种子发育中期延长WRI 1的表达显著提高了种子含油量。这项研究表明，参与油或贮藏蛋白生物合成的基因的时间激活决定了拟南芥种子中的油/蛋白质比。这些结果为潜在的育种策略提供了新的见解，以产生种子含油量高的作物。
Regulation of oil biosynthesis in plant seeds has been extensively studied, and biotechnological approaches have been designed to increase seed oil content. Oil and protein synthesis is negatively correlated in seeds, but the mechanisms controlling interactions between these two pathways are unknown. Here, we identify the molecular mechanism controlling oil and protein content in seeds. We utilized transgenic Arabidopsis thaliana plants overexpressing WRINKLED1 (WRI1), a master transcription factor regulating seed oil biosynthesis, and knockout mutants of major seed storage proteins. Oil and protein biosynthesis in wild-type plants was sequentially activated during early and late seed development, respectively. The negative correlation between oil and protein contents in seeds arises from competition between the pathways. Extension of WRI1 expression during mid-phase of seed development significantly enhanced seed oil content. This study demonstrates that temporal activation of genes involved in oil or storage protein biosynthesis determines the oil/protein ratio in Arabidopsis seeds. These results provide novel insights into potential breeding strategies to generate crops with high oil contents in seeds.