Transcriptomic dissection underlying physiological and anatomical characteristics of poplar wood in response to changes in light intensity and nitrogen availability
Transcriptomic dissection underlying physiological and anatomical characteristics of poplar wood in response to changes in light intensity and nitrogen availability
复制标题
杨树生理和解剖特征对光强度和氮利用率变化的转录组解剖
DOI：
10.1016/j.envexpbot.2022.105186
复制
发表时间：
2022-12-16
影响因子：
5.7
通讯作者：
Luo，Zhi-Bin
中科院分区：
文献类型：
--
作者：
Zhu，Dongyue;Li，Zhuorong;Luo，Zhi-Bin
为研究杨树木材生理解剖特征对光照和氮素供应变化的响应，以杨树幼树为材料，研究了杨树木材生理解剖特征的转录组重编程。alba×P.腺体暴露于对照或高光强结合低，正常和高N水平之一。正常氮条件下，强光使杨树木材CO2同化速率增大，可溶性糖和淀粉含量升高，NR和GS酶活性降低，GOGAT和GDH活性升高，IAA含量升高，阿坝、JA和SA含量降低，纤维变窄，纤维壁变薄。低氮处理导致白杨木材中蔗糖和淀粉含量增加，大多数氨基酸、IAA、阿坝和JA含量降低，导管和纤维变窄，而高氮处理则相反。转录组重编程的发生是P. alba×P.高光照与低、正常和高N水平组合的腺体素驯化。强光和低/高氮诱导的中性转化酶ASN 1、JAZ 1和TIP 1;3等关键基因的差异表达与白杨木材生理和解剖特征的变化相一致，这些基因参与了碳水化合物、氨基酸和植物激素的代谢以及水分运输。这些结果表明，生理和解剖特征的改变，在木材和底层的转录组重编程是至关重要的木材在适应强光与变化的N可用性相结合。
To dissect the transcriptomic reprograming underlying the physiological and anatomical characteristics in the wood ofPopulusspecies in response to changes in light and nitrogen (N) availability, the saplings ofP. alba×P. glandulosawere exposed to either control or high light intensity in combination with one of low, normal and high N levels. High light caused greater CO2assimilation rate, higher concentrations of soluble sugars and starch, decreased enzymatic activities of NR and GS and increased activities of GOGAT and GDH, higher IAA and lower ABA, JA and SA contents, narrower fibers, and thinner thickness of fiber wall in the wood of poplars under normal N condition. Low N supply led to greater levels of sucrose and starch, lower contents of most amino acids, IAA, ABA and JA, and narrower vessels and fibers, and high N caused the opposite effects in poplar wood exposed to control light. Transcriptomic reprograming occurred underlying the physiological and anatomical changes ofP. alba×P. glandulosein acclimation to high light in combination with one of low, normal and high N levels. High light- and low/high N-induced differential expression of the key genes includingneutralinvertases,ASN1,JAZ1, andTIP1;3, involved in the metabolism of carbohydrate, amino acids and phytohormones, and water transport, corresponded well to changes in physiological and anatomical characteristics in poplar wood in response to high light and altered N availability. These results suggest that physiological and anatomical characteristics are altered in the wood and the underlying transcriptomic reprograming is critical for the wood in acclimation to high light in combination with changing N availability.