Defects in lens fiber differentiation are linked to c-mos overexpression in transgenic mice.
Defects in lens fiber differentiation are linked to c-mos overexpression in transgenic mice.
复制标题
转基因小鼠中晶状体纤维分化的缺陷与 c-mos 过度表达有关。
DOI：
10.1101/gad.1.10.1327
复制
发表时间：
1987
影响因子：
10.5
通讯作者：
Westphal,H
中科院分区：
文献类型：
--
作者：
Khillan,JS;Oskarsson,MK;Propst,F;Kuwabara,T;VandeWoude,GF;Westphal,H
我们描述了三个品系的转基因小鼠胚胎显微注射的DNA组成的长末端重复序列（LTR）的莫洛尼鼠肉瘤病毒（Mo-MSV）连接到小鼠的c-mos编码序列。这些菌株的基因组DNA的Southern分析表明，在每种情况下，转基因已整合在不同的染色体位置。菌株的特点是显性变化的次生透镜纤维分化。出生后不久，分化透镜纤维的伸长不足和基底膜分泌缺乏导致后透镜囊破裂。这反过来又导致透镜组织的后部突出和肿胀。在出生后的前3周内，球形透镜细胞开始充满眼睛的整个前房和后房。同时，在透镜中有大量的c-mos RNA过表达。尽管该构建体在转染到NIH-3 T3细胞中时具有高转化活性，但在受影响的晶状体中未观察到增生或瘤形成。c-mos RNA的表达增加并不局限于眼睛的透镜，但已在任何几种测试组织中检测到。
We describe three strains of transgenic mice derived by embryo microinjection of DNA consisting of a long terminal repeat (LTR) of Moloney murine sarcoma virus (Mo-MSV) linked to the murine c-mos coding sequences. Southern analysis of the genomic DNA of these strains suggested that in each case the transgene had integrated at a different chromosomal location. The strains were characterized by dominant changes in secondary lens fiber differentiation. Shortly after birth, insufficient elongation of differentiating lens fibers and lack of basement membrane secretion resulted in breakdown of the posterior lens capsule. This, in turn led to posterior protrusion and swelling of lens tissue. In the course of the first 3 weeks after birth, globular lens cells began to fill the entire anterior and posterior chambers of the eye. Concomitantly, there was massive overexpression of c-mos RNA in the lens. Whereas this construct has high transforming activity when transfected into NIH-3T3 cells, no hyperplasia or neoplasia have been observed in the affected lenses. Increased expression of c-mos RNA was not confined to the lens of the eye but has been detected in any of several tissues tested.