Circulating endothelial and endothelial progenitor cells in patients with severe sepsis
Circulating endothelial and endothelial progenitor cells in patients with severe sepsis
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DOI：
10.1016/j.mvr.2010.11.011
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发表时间：
2011-03-01
影响因子：
3.1
通讯作者：
Yan, Sau-Chi Betty
中科院分区：
文献类型：
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作者：
Schlichting, Douglas E.;Waxman, Aaron B.;Yan, Sau-Chi Betty
循环内皮细胞(CEC)和循环内皮祖细胞(CEPC)计数升高可能指示血管损伤和疾病状态，但关于严重脓毒症患者这些细胞数量的数据有限。这项研究比较了重症监护病房(ICU)入院后有严重脓毒症和无严重脓毒症的患者的CEC和CEPC计数。分别在24 h、48-72 h和120-144 h内采集静脉血，记录基线人口学、28天病死率、ICU天数、住院天数和序贯器官衰竭评估(SOFA)评分。严重脓毒症患者(n=18)和非严重脓毒症患者(n=28)的平均年龄(分别为63.7岁和61.3岁)和性别进行了平衡。在28天死亡率、ICU天数或住院天数方面没有差异。脓毒症组的基线SOFA评分较高。在48-72小时内，严重脓毒症患者CEC计数的中位数显著高于对照组(51.5比28.0细胞/4ml血，P=0.02)。所有ICU患者的CEC值在任何时间点均无显著差异(P21菌落/4ml血(平均值=1.13+/-2.25；中位数=0)。这项研究展示了使用共识方法对CEC和CEPC进行量化的能力。了解CEC/CEPC计数和预后之间的关系可能有助于深入了解严重脓毒症时内皮细胞变化的机制。(C)2010 Elsevier Inc.保留所有权利。
Elevated circulating endothelial cell (CEC) and circulating endothelial progenitor cell (CEPC) counts may indicate vascular damage and disease status, but data on these cell populations in patients with severe sepsis are limited. This study compared CEC and CEPC counts in patients with and without severe sepsis following intensive care unit (ICU) admission. Venous blood samples were collected within 24 h, 48-72 h, and 120-144 h. Baseline demographics, 28-day mortality, ICU and hospital days, and Sequential Organ Failure Assessment (SOFA) scores were recorded. Patients with (n = 18) and without (n = 28) severe sepsis were balanced for mean age (63.7 and 61.3 years, respectively) and gender. There were no differences in 28-day mortality, ICU days, or hospital days. Baseline SOFA scores were higher in the sepsis group. At 48-72 h, patients with severe sepsis had significantly higher median CEC counts (51.5 vs. 28.0 cells/4 ml of blood, P=0.02). CEC values for all ICU patients were significantly(P21 colonies/4 ml blood (mean = 1.13 +/- 2.25; median = 0) without significant differences at any time point. This study demonstrates the ability to quantify CECs and CEPCs using consensus methodology. Understanding the relationship between CEC/CEPC counts and outcomes may provide insight into the mechanisms of endothelial cell changes in severe sepsis. (C) 2010 Elsevier Inc. All rights reserved.