The role of cathepsin D in the pathogenesis of tuberculosis. A histochemical study employing unlabeled antibodies and the peroxidase-antiperoxidase complex.
The role of cathepsin D in the pathogenesis of tuberculosis. A histochemical study employing unlabeled antibodies and the peroxidase-antiperoxidase complex.
复制标题
组织蛋白酶 D 在结核病发病机制中的作用。
作者：
O. Rojas;A. Dannenberg;L. Sternberger;T. Tsuda
组织蛋白酶D在游离肺泡巨噬细胞（AM），油诱导的腹腔巨噬细胞（MN）和兔肺和皮肤BCG病变与未标记的抗体和过氧化物酶-抗过氧化物酶（PAP）复合物可视化。组织蛋白酶D在AM中大量存在，而在MN中含量较低。在肺中，这种酶在积聚在BCG损伤周围的肺泡巨噬细胞中最丰富。在真皮病变中，组织蛋白酶D在坏死（液化）中心边缘的巨噬细胞中浓度最高。在真皮上皮和毛囊的角化细胞中也发现了高浓度。然而，在许多对溶酶体酶β-半乳糖苷酶染色强烈的活化巨噬细胞中，它并没有明显增加。事实上，许多具有高β-半乳糖苷酶活性的上皮样细胞不含可见的组织蛋白酶D。因此，这种蛋白酶似乎并不主要参与淋巴细胞介导的巨噬细胞活化，而巨噬细胞活化与获得性细胞对结核杆菌的耐药性有关。它可能更多地涉及细胞自溶，消化摄入的坏死碎片，并且很可能涉及液化过程，这是人类结核病发病机制中最不利的事件。
Cathepsin D was visualized in free pulmonary alveolar macrophages (AM), in oil-induced peritoneal macrophages (MN) and in rabbit pulmonary and dermal BCG lesions with unlabeled antibodies and the peroxidase-antiperoxidase (PAP) complex. Large amounts of cathepsin D were present in AM and lower amounts in MN. In the lung this enzyme was richest in the alveolar macrophages that accumulated around the BCG lesions. In the dermal lesions, cathepsin D was in highest concentration in macrophages at the border of the necrotic (liquefying) centers. It was also found in high concentration in keratinizing cells of the dermal epithelium and hair follicles. It did not, however, increase appreciably in many of the activated macrophages that stained intensely for the lysosomal enzyme beta-galactosidase. In fact, many epithelioid cells with high beta-galactosidase activity contained no visible cathepsin D. This proteinase does not, therefore, seem to be primarily involved in the lymphocyte-mediated macrophage activation associated with acquired cellular resistance to tubercle bacilli. It is probably more involved with cell autolysis, with the digestion of ingested necrotic debris and, in all likelihood, with the process of liquefaction, the most adverse event in the pathogenesis of tuberculosis in man.