Cytomegalovirus blood viral load and hearing loss in young children with congenital infection.
Cytomegalovirus blood viral load and hearing loss in young children with congenital infection.
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DOI：
10.1097/inf.0b013e3181979a27
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
Boppana SB
中科院分区：
文献类型：
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作者：
Ross SA;Novak Z;Fowler KB;Arora N;Britt WJ;Boppana SB
本研究旨在确定婴幼儿病毒载量升高是否与先天性巨细胞病毒（CMV）相关性听力损失相关。从135名先天性CMV感染的儿童中获得血液样本。外周血中的CMV DNA定量与实时聚合酶链反应测定。在3个不同年龄组（<2个月、2-12个月、12-36个月）中分析病毒载量测量。在有症状和无症状感染的儿童中，所有3个年龄组中听力障碍儿童和听力正常儿童的CMV DNA水平没有差异。在无症状感染的儿童中，外周血病毒载量<3500基因组当量/毫升（ge/mL）的阳性预测值在<2个月和2至12个月时为8%，在12至36个月时为11.8%。然而，病毒载量<3500 ge/mL的阴性预测值在<2月龄时为94.4%，在2至36月龄时为100%。先天性巨细胞病毒感染患儿外周血病毒载量与听力损失无关然而，病毒载量<3500 ge/mL与出生时无症状先天性感染的儿童听力损失风险较低相关。
This study was designed to determine whether elevated viral load in infants and young children is associated with congenital cytomegalovirus (CMV)-related hearing loss. Blood samples were obtained from 135 children with congenital CMV infection. CMV DNA in the peripheral blood was quantitated with a real-time polymerase chain reaction assay. Viral load measurements were analyzed in 3 different age groups (<2 months, 2–12 months, 12–36 months). In children with symptomatic and asymptomatic infection, CMV DNA levels were not different between children with hearing deficit and those with normal hearing in all 3 age groups. In children with asymptomatic infection, the positive predictive value of a peripheral blood viral load <3500 genomic equivalents per milliliter (ge/mL) at <2 months and 2 to 12 months of age is 8%, and at 12 to 36 months of age is 11.8%. However, the negative predictive value of a viral load <3500 ge/mL is 94.4% at <2 months of age, and 100% at 2 to 36 months of age. Peripheral blood viral load is not associated with hearing loss in children with congenital CMV infection. However, a viral load of <3500 ge/mL is associated with a lower risk of hearing loss in children born with asymptomatic congenital infection.