Small ribonucleoprotein particle protein SmD3 governs the homeostasis of germline stem cells and the crosstalk between the spliceosome and ribosome signals in Drosophila
Small ribonucleoprotein particle protein SmD3 governs the homeostasis of germline stem cells and the crosstalk between the spliceosome and ribosome signals in Drosophila
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小核糖核蛋白颗粒蛋白 SmD3 控制果蝇生殖干细胞的稳态以及剪接体和核糖体信号之间的串扰
DOI：
10.1096/fj.201802536rr
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Fang Jie
中科院分区：
文献类型：
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作者：
Yu Jun;Luan Xiaojin;Yan Yidan;Qiao Chen;Liu Yuanyuan;Zhao Dan;Xie Bing;Zheng Qianwen;Wang Min;Chen Wanyin;Shen Cong;He Zeyu;Hu Xing;Huang Xiaoyan;Li Hong;Chen Binghai;Zheng Bo;Chen Xia;Fang Jie
真核生物中核糖核蛋白（RNP）剪接体机制触发前体RNA剪接过程。主要的剪接体缺陷与雄性不育有关;然而，果蝇睾丸中剪接体和核糖体之间的潜在机制联系在很大程度上仍未得到解决。小核糖核蛋白颗粒蛋白SmD 3（SmD 3）是我们在前期果蝇睾丸筛选中发现的一个新的生殖系干细胞（GSC）调控基因。在本研究中，使用果蝇模型中的遗传操作，我们证明SmD 3是GSC生态位所需的，并控制睾丸中GSC的自我更新和分化。在施耐德2细胞体外试验中，我们发现，SmD 3也调节果蝇的增殖和凋亡的稳态。此外，使用液相色谱-串联质谱法，SmD 3被确定为与核糖体蛋白（Rp）L18，这是一个关键的调节大亚基的核糖体结合。此外，SmD 3被观察到调节剪接体和核糖体亚单位的表达水平，并通过RpL 18控制剪接体和核糖体功能。值得注意的是，我们的研究结果揭示了干细胞生态位以及剪接体和核糖体之间串扰的遗传原因和分子机制。余，J.，Luan，X.，Yan，Y.，Qiao，C，Liu，Y.，赵，D.，Xie，B.，郑昆，王，M.，陈伟，Shen，C，He，Z.，Hu，X.，黄，X.，Li，H.，Chen，B.，Zheng，B.，陈旭，Fang，J. Small ribonucleoprotein particle protein SmD3 governs the homeostasis of germline stem cells and the crosstalk between the spliceosome and ribosome signals in Drosophila. FASEB J. 33，8125-8137（2019）。www.fasebj.org
The ribonucleoprotein (RNP) spliceosome machinery triggers the precursor RNA splicing process in eukaryotes. Major spliceosome defects are implicated in male infertility; however, the underlying mechanistic links between the spliceosome and the ribosome in Drosophila testes remains largely unresolved. Small ribonucleoprotein particle protein SmD3 (SmD3) is a novel germline stem cell (GSC) regulatory gene identified in our previous screen of Drosophila testes. In the present study, using genetic manipulation in a Drosophila model, we demonstrated that SmD3 is required for the GSC niche and controls the self‐renewal and differentiation of GSCs in the testis. Using in vitro assays in Schneider 2 cells, we showed that SmD3 also regulates the homeostasis of proliferation and apoptosis in Drosophila. Furthermore, using liquid chromatography–tandem mass spectrometry methods, SmD3 was identified as binding with ribosomal protein (Rp)L18, which is a key regulator of the large subunit in the ribosome. Moreover, SmD3 was observed to regulate spliceosome and ribosome subunit expression levels and controlled spliceosome and ribosome function via RpL18. Significantly, our findings revealed the genetic causes and molecular mechanisms underlying the stem cell niche and the crosstalk between the spliceosome and the ribosome.—Yu, J., Luan, X., Yan, Y., Qiao, C, Liu, Y., Zhao, D., Xie, B., Zheng, Q., Wang, M., Chen, W., Shen, C, He, Z., Hu, X., Huang, X., Li, H., Chen, B., Zheng, B., Chen, X., Fang, J. Small ribonucleoprotein particle protein SmD3 governs the homeostasis of germline stem cells and the crosstalk between the spliceosome and ribosome signals in Drosophila. FASEB J. 33, 8125–8137 (2019). www.fasebj.org