Protein polymorphism generated by differential RNA editing of a plant mitochondrial rps12 gene.
Protein polymorphism generated by differential RNA editing of a plant mitochondrial rps12 gene.
复制标题
植物线粒体 rps12 基因差异 RNA 编辑产生的蛋白质多态性。
DOI：
10.1128/mcb.16.4.1543
复制
发表时间：
1996
影响因子：
5.3
通讯作者：
Hanson,MR
中科院分区：
文献类型：
--
作者：
Lu,B;Wilson,RK;Phreaner,CG;Mulligan,RM;Hanson,MR
Therps12gene transcripts encoding mitochondrial ribosomal protein S12 are partially edited in petunia mitochondria. Different petunia lines were found vary in the extent ofrps12transcript editing. To test whether multiple forms of RPS12 proteins are produced in petunia mitochondria as a result of partial editing, we probed mitochondrial proteins with specific antibodies against edited and unedited forms of a 13-amino-acid RPS12 peptide spanning two amino acids affected by RNA editing. Both antibodies reacted with mitochondrial proteins at the expected size for RPS12 proteins. The amounts of unedited RPS12 protein in different petunia lines correlate with the abundance of unedited transcripts in these plants. Uneditedrps12translation products are also detected in other plant species, indicating that polymorphism in mitochondrialrps12expression is widespread. Moreover, we show that RPS12 proteins recognized by both edited-specific and unedited-specific antibodies are present in a petunia mitochondrial ribosome fraction. These results demonstrate that partially edited transcripts can be translated and that the protein product can accumulate to detectable levels. Therefore, genes exhibiting incompletely edited transcripts can encode more than one gene product in plant mitochondria.
