Networks of protein-protein interactions among structural proteins of budded virus of Bombyx mori nucleopolyhedrovirus
Networks of protein-protein interactions among structural proteins of budded virus of Bombyx mori nucleopolyhedrovirus
复制标题
家蚕核多角体病毒芽生病毒结构蛋白之间的蛋白质-蛋白质相互作用网络
DOI：
10.1016/j.virol.2018.02.015
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Wu Xiaofeng
中科院分区：
文献类型：
--
作者：
Zhang Jianjia;Feng Min;Fan Ying;Xu Weifan;Zheng Qin;Wu Xiaofeng
杆状病毒的结构蛋白已经得到了很好的研究，但它们之间的相互作用尚不清楚。为了揭示家蚕核型多角体病毒(BmNPV)芽生病毒结构蛋白及其相关蛋白之间的蛋白质-蛋白质相互作用，利用酵母双杂交(Y2H)系统对27个病毒基因产物的相互作用进行了评价。共鉴定出57个相互作用，其中51个是二元相互作用，6个是自关联。免疫共沉淀法进一步证实了其中的10个相互作用。通过直接交叉Y2H分析，形成了5个相互作用网络。VP39、38 K和FP与大多数病毒蛋白相互作用，并可能形成病毒结构的主要结构元素。此外，检测到每个包膜蛋白与一个以上的衣壳蛋白相互作用。这些结果表明，病毒结构蛋白和结构相关蛋白如何通过相互作用组装成完整的病毒。
The structural proteins of baculovirus are well studied, but the interactions between them remain unclear. In order to reveal protein-protein interactions among viral structural proteins and their associated proteins of the budded virus ofBombyx morinucleopolyhedrovirus (BmNPV), the yeast two hybrid (Y2H) system was used to evaluate the interactions of 27 viral genes products. Fifty-seven interactions were identified with 51 binary interactions and 6 self-associations. Among them, 10 interactions were further confirmed by co-immunoprecipitation assays. Five interaction networks were formed based on the direct-cross Y2H assays. VP39, 38 K, and FP were identified to interact with most of the viral proteins, and may form major structural elements of the viral architecture. In addition, each envelope protein was detected to interact with more than one capsid protein. These results suggest how viral structural and structural associated proteins may assemble to form a complete virus through interacting with each other.