Photoreceptor apoptosis in human retinal detachment
Photoreceptor apoptosis in human retinal detachment
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DOI：
10.1016/j.ajo.2004.11.046
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发表时间：
2005-04-01
影响因子：
4.2
通讯作者：
Chen, DF
中科院分区：
文献类型：
--
作者：
Arroyo, JG;Yang, L;Chen, DF
目得：虽然感光细胞凋亡已被证明在各种动物模型的视网膜脱离（RD），很少有人知道它在人类RD的发生。我们试图确定人类感光细胞是否会发生凋亡，以应对原发性和复发性RD.DESIGN：前瞻性，临床病理，cases series.METHODS：视网膜组织片段切除过程中的玻璃体手术RD和复发性RD被冷冻，切成4 μ m的部分，并分析使用TdT-dUTP末端缺口末端标记细胞凋亡测定。患者症状的发作被用来估计的RD.RESULTS的持续时间：有8例原发性RD和4例复发性RD患者参加了这项研究。原发性RD组的RD持续时间为1 - 180天，复发性RD组为2 - 15天。所有视网膜组织标本均具有TUNEL阳性细胞，定位于视网膜的外核层，与感光细胞体的定位一致。TUNEL阳性细胞在RD后24小时首次被鉴定，在RD后2天达到峰值，并在RD后7天降至低水平。复发性RD诱导ONL中TUNEL阳性细胞数/mm（2）与RD发作后相应时间点的原发性RD相比。结论：在对原发性和复发性D的反应中，人类感光细胞遵循与RD动物模型中所见相似的凋亡模式。本研究提示视网膜光感受器细胞凋亡可能是视网膜脱离后视力下降的原因之一，尤其是黄斑区。抑制感光细胞凋亡的药物可能有助于改善RD患者的最终视力预后。(Am J Ophthalmol 2005;139：605-610. (c)2005年，Elsevier Inc.保留所有权利。
PURPOSE: Although photoreceptor cell apoptosis has been demonstrated in various animal models of retinal detachment (RD), little is known about its occurrence in human RD. We sought to determine whether human photoreceptor cells undergo apoptosis in response to primary and recurrent RD.DESIGN: Prospective, clinical-pathologic, case series.METHODS: Retinal tissue fragments excised during the course of vitreous surgery for RD and recurrent RD were frozen, cut into 4-mu m sections, and analyzed using a TdT-dUTP terminal nick-end labeling assay for cell apoptosis. The onset of patient symptoms was used to estimate the duration of the RD.RESULTS: There were eight patients with primary RD and four patients with recurrent RD enrolled in this study. Duration of RD ranged from 1 to 180 days in the primary RD group, and 2 to 15 days in the recurrent RD group. All retinal tissue specimens had TUNEL,positive cells localized to the outer nuclear layer of the retina, consistent with the localization of photoreceptor cell bodies. TUNEL-positive cells were first identified at 24 hours, peaked by 2 days, and dropped to a low level by 7 days after RD. Recurrent RD induced a greater number of TUNEL-positive cells/mm(2) in the ONL compared with primary RD at corresponding timepoints after the onset of RD.CONCLUSIONS: In response to primary and recurrent D, human photoreceptor cells follow a pattern of apoptosis that is similar to that seen in animal models of RD. This study suggests that photoreceptor cell apoptosis may be one of the causes of reduced vision after RD, especially those that involve the macula. Drugs that inhibit photoreceptor apoptosis may help improve the final visual prognosis of patients with RD. (Am J Ophthalmol 2005;139:605-610. (c) 2005 by Elsevier Inc. All rights reserved).