Impact of soil salinity on the microbial structure of halophyte rhizosphere microbiome
Impact of soil salinity on the microbial structure of halophyte rhizosphere microbiome
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DOI：
10.1007/s11274-018-2509-5
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发表时间：
2018-09-01
影响因子：
4.1
通讯作者：
Malik, Kauser Abdulla
中科院分区：
文献类型：
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作者：
Mukhtar, Salma;Mirza, Babur Saeed;Malik, Kauser Abdulla
根际微生物组在植物的生活中起着重要的作用，促进植物在不利条件下的存活。然而，有限的信息是关于微生物多样性在盐水环境。在目前的研究中，我们比较了根际微生物的组成的盐生植物尾藻，地肤，猪毛菜，和滨藜生活在中等和高盐度的环境（Khewra盐矿，巴基斯坦）与非盐生植物小麦。利用16 S rRNA基因焦磷酸测序技术对土壤微生物群落进行分析表明，盐渍土中放线菌占优势，非盐渍土中变形菌占优势。厚壁菌门、酸杆菌门、拟杆菌门和Thaumarchaeota门是盐渍土和非盐渍土中的优势门，而蓝细菌门、疣微菌门、芽单胞菌门和未分类的WPS-2门的丰度较低。来自Euryarchaeota，Ignavibacteriae和Nanohaloarchaeota的序列仅从盐生植物的根际中确定。结果表明，该地区的优势嗜盐菌和古菌包括Agrococcus、Armatimonadetes gp 4、Halalkalicoccus、Haloferula和Halobacterium。我们的分析表明，土壤盐分的增加与盐渍土和非盐渍土样品中微生物群落的α和β多样性的显着差异相关。对巴基斯坦不同盐碱环境中的土壤细菌进行完整的清点，将有助于发现在受盐碱影响的土地上种植作物的潜在接种剂。
The rhizosphere microbiome plays a significant role in the life of plants in promoting plant survival under adverse conditions. However, limited information is available about microbial diversity in saline environments. In the current study, we compared the composition of the rhizosphere microbiomes of the halophytes Urochloa, Kochia, Salsola, and Atriplex living in moderate and high salinity environments (Khewra salt mines; Pakistan) with that of the non-halophyte Triticum. Soil microbiomes analysis using pyrosequencing of 16S rRNA gene indicated that Actinobacteria were dominant in saline soil samples whereas Proteobacteria predominated in non-saline soil samples. Firmicutes, Acidobacteria, Bacteriodetes and Thaumarchaeota were predominant phyla in saline and non-saline soils, whereas Cyanobacteria, Verrucomicrobia, Gemmatimonadetes and the unclassified WPS-2 were less abundant. Sequences from Euryarchaeota, Ignavibacteriae, and Nanohaloarchaeota were identified only from the rhizosphere of halophytes. Dominant halophilic bacteria and archaea identified in this study included Agrococcus, Armatimonadetes gp4, Halalkalicoccus, Haloferula and Halobacterium. Our analysis showed that increases in soil salinity correlated with significant differences in the alpha and beta diversity of the microbial communities across saline and non-saline soil samples. Having a complete inventory of the soil bacteria from different saline environments in Pakistan will help in the discovery of potential inoculants for crops growing on salt-affected land.