A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS
A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS
复制标题
DOI：
10.1016/0008-6215(83)88244-5
复制
发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
STONE, BA
中科院分区：
文献类型：
--
作者：
BLAKENEY, AB;HARRIS, PJ;STONE, BA
介绍了一种由单糖制备糖醇乙酸酯的简便、快速的方法。它可以在单个管中进行，而无需转移或蒸发。单糖在二甲亚砜中用硼氢化钠还原，所得糖醇使用1-甲基咪唑作为催化剂乙酰化。在室温下，乙酰化反应10 min即可完成，不需要除去硼酸根.单糖被定量地还原和乙酰化。糖醇乙酸酯通过玻璃毛细管、GLC和Silar 10 C完全分离。将该方法应用于植物[Lolium multiflorum]细胞壁酸水解物中单糖的分析。
A simple and rapid method is described for the preparation of alditol acetates from monosaccharides. It can be performed in a single tube without transfers or evaporations. Monosaccharides are reduced with sodium borohydride in dimethyl sulfoxide and the resulting alditols acetylated using 1-methylimidazole as the catalyst. Removal of borate is unnnecessary and acetylation is complete in 10 min at room temperature. Monosaccharides are quantitatively reduced and acetylated by this procedure. The alditol acetates are completely separated by glass-capillary, GLC an Silar 10C. The method was applied to the analysis of monosaccharides in acid hydrolysates of a plant [Lolium multiflorum] cell-wall.