Proglumide inhibition of trophic action of pentagastrin.
Proglumide inhibition of trophic action of pentagastrin.
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丙谷胺抑制五肽胃泌素的营养作用。
DOI：
10.1152/ajpgi.1984.246.1.g62
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Guthrie,PD
中科院分区：
文献类型：
--
作者：
Johnson,LR;Guthrie,PD
丙谷氨酰胺是一种谷氨酸衍生物，抑制胃泌素的酸分泌作用，据说是一种特异性的胃泌素受体阻断剂。在目前的研究中，我们发现丙氨酸抑制了大鼠氧合腺粘膜中胃泌素与其受体的结合，并测试了该受体是否也与胃泌素的营养作用有关。在第一项研究中，400 mg/kg丙氨酸每8 h注射250 mg/kg戊胃泌素，连续48 h完全阻止了戊胃泌素对大鼠氧合腺粘膜的营养作用。测量的参数包括DNA合成和DNA、RNA和蛋白质含量。在第二项研究中，确定丙氨酰胺的最低最大有效剂量为100 mg/kg。胃泌素对十二指肠黏膜、结肠黏膜、胰腺及氧合腺粘膜的营养作用均受到抑制。这些数据表明丙氨酸阻断了外源性胃泌素的营养作用，表明胃泌素的营养作用是由胃泌素受体介导的。
Proglumide, a glutaramic acid derivative, inhibits the acid secretory effects of gastrin and is said to be a specific gastrin receptor blocking agent. In the current study we have shown that proglumide inhibits the binding of gastrin to its receptor in rat oxyntic gland mucosa and tested whether this receptor is also responsible for the trophic effect of gastrin. In the first study 400 mg/kg proglumide injected with 250 micrograms/kg pentagastrin every 8 h for 48 h totally prevented the trophic effects of pentagastrin in rat oxyntic gland mucosa. Parameters measured included DNA synthesis and DNA, RNA, and protein content. In a second study the lowest maximally effective dose of proglumide was determined to be 100 mg/kg. The trophic effect of pentagastrin was inhibited in duodenal mucosa, colonic mucosa, and pancreas as well as oxyntic gland mucosa. These data demonstrate that proglumide blocks the trophic action of exogenous gastrin and suggest that the trophic effect of gastrin is mediated by the gastrin receptor.