Direct detection of potassium and lead (II) ions based on assembly- disassembly of a chiral cyanine dye /TBA complex
Direct detection of potassium and lead (II) ions based on assembly- disassembly of a chiral cyanine dye /TBA complex
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基于手性花青染料/TBA 复合物的组装-分解直接检测钾和铅 (II) 离子
DOI：
10.1016/j.talanta.2019.04.032
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Tang Yalin
中科院分区：
文献类型：
--
作者：
Shen Gang;Zhang Hong;Xiang Junfeng;Yang Fengmin;Wu Shuling;Wang Wei;Du Ning;Zhang Jinghua;Sun Tao;Tang Yalin
利用手性菁染料/TBA配合物的组装和拆分，建立了一种高选择性、高灵敏度的钾（K+）和铅（Pb 2+）的直接检测方法。染料DMSB（3-乙基-2-[3-（3-乙基-3H-苯并硒唑-2-亚基）-2-甲基丙-1-烯基]苯并硒唑溴化物）失去了自组装能力，但它可以被凝血酶结合适体（TBA）G-四链体结构激活。只有在K+存在下形成的TBA G-四链体才能强烈诱导DMSB的J-聚集信号。由于Pb ~（2+）离子能以比K ~+高得多的效率结合和稳定TBA G-四链体，所以当Pb ~（2+）存在时，DMSB的J-聚集体信号急剧下降。结果表明，DMSB的组装和拆卸使其能够分别选择性地检测10 μM K+和20 nM Pb 2+，即使竞争性钠离子（Na+）高达145 mM。K+和Pb 2+浓度分别在0.5-5.0 mM和200-2000 nM范围内与J-聚集体强度呈线性相关。此外，通过本方法测定人血清样品中K+（约3 mM）和Pb 2+（低于20 nM）的浓度，其与电感耦合等离子体质谱（ICP-MS）一致。本工作不仅为基于G-四链体的适配器在复杂真实的体系中的进一步开发打开了一扇大门，而且为临床离子检测提供了一个简单、通用的传感平台。
A highly selective and sensitive direct detection of potassium (K+) and lead (Pb2+) ions was developed by using the assembly and disassembly of a chiral cyanine dye/TBA complex. The dyeDMSB(3-ethyl-2-[3-(3-ethyl-3H-benzoselenazol-2-ylidene)-2-methylprop-1-enyl] benzoselenazolium bromide) loses the ability of self-assembly, but it can be activated by thrombin-binding aptamer (TBA) G-quadruplex structure. And only the TBA G-quadruplex formed in the presence of K+, can strongly induce J-aggregate signals ofDMSB. Because the Pb2+ions can bind and stabilize the TBA G-quadruplex with much higher efficiency than K+, the J-aggregate signals ofDMSBfalls sharply when the Pb2+is present. As a result, the assembly and disassembly ofDMSBallows the selective detection of 10 μM K+and 20 nM Pb2+respectively, even the competitive sodium ion (Na+) was as high as 145 mM. The linear correlation existed between the J-aggregate intensity and the concentration of K+and Pb2+over the range of 0.5–5.0 mM and 200–2000 nM, respectively. Moreover, the concentration of K+(∼3 mM) and Pb2+(below 20 nM) in human blood serum samples were determined by the present method, which agreed well with inductively coupled plasma mass spectrometry (ICP-MS). This work not only opens a door for the further development of G-quadruplex-based aptasensor in complex real system, but also provides a simple and versatile sensing platform for ion detection in clinic.