The basidiomycete Lentinus edodes linear mitochondrial DNA plasmid contains a segment exhibiting a high autonomously replicating sequence activity in Saccharomyces cerevisiae.
The basidiomycete Lentinus edodes linear mitochondrial DNA plasmid contains a segment exhibiting a high autonomously replicating sequence activity in Saccharomyces cerevisiae.
复制标题
担子菌香菇线性线粒体 DNA 质粒含有在酿酒酵母中表现出高自主复制序列活性的片段。
DOI：
10.1093/nar/18.6.1395
复制
发表时间：
1990
影响因子：
14.9
通讯作者：
K. Shishido
中科院分区：
文献类型：
--
作者：
Y. Katayose;S. Kajiwara;K. Shishido
从香菇（Lentinus edodes）菌株的线粒体组分中分离到一个线性DNA质粒，命名为pLLE 1。pLLE 1（11.0kbp）对3 ′-5 ′-作用的外切核酸酶III敏感，对5 ′-3 ′-作用的λ外切核酸酶有抗性。与无质粒株及携带pLLE 1的宿主菌线粒体和核基因组DNA均无同源性。edodes。用YIp 32（含酵母LEU 2 DNA的pBR 322）从pLLE 1 DNA中克隆了一个1434 bp的能在酵母酿酒酵母（Saccharomycescerevisiae，ARS）中自主复制的片段（序列），该片段具有较高的ARS活性。克隆的1434 bp片段位于pLLE 1 DNA的末端（约800-2200个核苷酸），含有三个连续的S的ARS共有序列（A/T）TTTAT（A/G）TTT（A/T）。cerevisiae和分散的八个ARS共识样序列。亚克隆的366-bp片段含有三个ARS保留原来的ARS活性的1434-bp片段。
A linear DNA plasmid, designated pLLE1, has been isolated from a mitochondrial fraction of a strain of Lentinus edodes. pLLE1(11.0 kbp) was sensitive to the 3'----5'-acting exonuclease III and resistant to the 5'----3'-acting lambda exonuclease. It showed no homology with mitochondrial and nuclear genomic DNAs of plasmidless strain as well as the pLLE1-harboring host strain of L. edodes. The 1434-bp fragment (sequences) capable of autonomous replication in the yeast Saccharomyces cerevisiae (ARSs) was cloned from pLLE1 DNA with YIp32 (pBR322 containing yeast LEU2 DNA), which displayed a high ARS activity. The cloned 1434-bp fragment was shown to lie near to the end of pLLE1 DNA (nucleotides about 800-2200) and contained three consecutive ARS consensus sequences (A/T)TTTAT(A/G)TTT(A/T) of S. cerevisiae and dispersive eight ARS consensus-like sequences. The subcloned 366-bp fragment containing the three ARSs retained original ARS activity of the 1434-bp fragment.