Novel Functional Genomics Approaches: A Promising Future in the Combat Against Plant Viruses
Novel Functional Genomics Approaches: A Promising Future in the Combat Against Plant Viruses
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DOI：
10.1094/phyto-03-16-0145-fi
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发表时间：
2016-10-01
期刊：
影响因子：
3.2
通讯作者：
Dinesh-Kumar, Savithramma P.
中科院分区：
文献类型：
--
作者：
Fondong, Vincent N.;Nagalakshmi, Ugrappa;Dinesh-Kumar, Savithramma P.
功能基因组学和基因组编辑方法的进展为通过以精确和可预测的方式修饰宿主和病毒基因组来加速植物病毒控制工作提供了新的机会和潜力。在这里，我们讨论了基于RNA的技术的应用，包括人工微RNA，transacting小干扰RNA和Cas9（成簇规则间隔短回文重复相关蛋白9），这些技术目前正在成功地用于产生抗病毒植物。我们进一步讨论了反向遗传学方法，靶向诱导基因组局部病变（TILLING）及其变体，称为EcoTILLING，用于鉴定植物病毒隐性抗性基因等位基因。除了描述这些技术在植物病毒控制中的具体应用外，本文还讨论了它们的优点和局限性。
Advances in functional genomics and genome editing approaches have provided new opportunities and potential to accelerate plant virus control efforts through modification of host and viral genomes in a precise and predictable manner. Here, we discuss application of RNA based technologies, including artificial micro RNA, transacting small interfering RNA, and Cas9 (clustered regularly interspaced short palindromic repeat-associated protein 9), which are currently being successfully deployed in generating virus-resistant plants. We further discuss the reverse genetics approach, targeting induced local lesions in genomes (TILLING) and its variant, known as EcoTILLING, that are used in the identification of plant virus recessive resistance gene alleles. In addition to describing specific applications of these technologies in plant virus control, this review discusses their advantages and limitations.