Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes.
Extragenic suppressors of Saccharomyces cerevisiae prp4 mutations identify a negative regulator of PRP genes.
复制标题
酿酒酵母 prp4 突变的基因外抑制因子识别出 PRP 基因的负调节因子。
DOI：
10.1093/genetics/136.3.833
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
WoolfordJr,JL
中科院分区：
文献类型：
--
作者：
Maddock,JR;Weidenhammer,EM;Adams,CC;Lunz,RL;WoolfordJr,JL
The PRP4 gene encodes a protein that is a component of the U4/U6 small nuclear ribonucleoprotein particle and is necessary for both spliceosome assembly and pre-mRNA splicing. To identify genes whose products interact with the PRP4 gene or gene product, we isolated second-site suppressors of temperature-sensitive prp4 mutations. We limited ourselves to suppressors with a distinct phenotype, cold sensitivity, to facilitate analysis of mutants. Ten independent recessive suppressors were obtained that identified four complementation groups, spp41, spp42, spp43 and spp44 (suppressor of prp4, numbers 1-4). spp41-spp44 suppress the pre-mRNA splicing defect as well as the temperature-sensitive phenotype of prp4 strains. Each of these spp mutations also suppresses prp3; spp41 and spp42 suppress prp11 as well. Neither spp41 nor spp42 suppressors null alleles of prp3 or prp4, indicating that the suppression does not occur via a bypass mechanism. The spp41 and spp42 mutations are neither allele- nor gene-specific in their pattern of suppression and do not result in a defect in pre-mRNA splicing. Thus the SPP41 and SPP42 gene products are unlikely to participate directly in mRNA splicing or interact directly with Prp3p or Prp4p. Expression of PRP3-lacZ and PRP4-lacZ gene fusions is increased in spp41 strains, suggesting that wild-type Spp41p represses expression of PRP3 and PRP4. SPP41 was cloned and sequenced and found to be essential. spp43 is allelic to the previously identified suppressor srn1, which encodes a negative regulator of gene expression.
