Mesoderm induction by BMP-4 and -7 heterodimers.
Mesoderm induction by BMP-4 and -7 heterodimers.
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BMP-4 和-7 异二聚体诱导中胚层。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
N. Ueno
中科院分区：
文献类型：
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作者：
A. Suzuki;Eiji Kaneko;J. Maeda;N. Ueno
骨形态发生蛋白 (BMP) 是属于 TGF-β 超家族的肽生长因子。大量这些配体，包括 BMP-2、-4 和 -7 在脊椎动物胚胎的早期胚胎发生过程中表达。在这项研究中，我们证明 BMP-7 在外胚层外植体和整个胚胎中都具有腹侧活性。与 BMP-2 和 BMP-4 的情况一样，当 BMP-7 作为同二聚体蛋白提供时，它是一种非常差的诱导剂。由于这种弱的中胚层诱导活性，有人认为 BMP 诱导的中胚层可能是过度表达的产物。我们提供的证据表明，与 BMP-4 或 BMP-7 的同二聚体不同，非洲爪蟾 BMP-4 和 BMP-7 的纯化重组异二聚体 (BMP-4/7) 在生理浓度下具有有效的中胚层诱导活性。这些结果为脊椎动物胚胎中 BMP-4/7 异二聚体的胚胎功能提供了第一个证据。
Bone morphogenetic proteins (BMPs) are peptide growth factors belonging to the TGF-beta superfamily. A large number of these ligands, including BMP-2, -4 and -7 is expressed during early embryogenesis in the vertebrate embryo. In this study, we demonstrate that BMP-7 has ventralizing activity both in ectodermal explants as well as in whole embryos. As it was the case for BMP-2 and BMP-4, BMP-7 is a very poor inducer when provided as a homodimer protein. Because of this weak mesoderm inducing activity, it has been suggested that mesoderm induction by BMPs might represent an artifact of overexpression. We provide evidence demonstrating that unlike the homodimers of BMP-4 or BMP-7, the purified recombinant heterodimer of Xenopus BMP-4 and BMP-7 (BMP-4/7) has a potent mesoderm inducing activity at physiological concentrations. These results provide the first evidence for an embryonic function of BMP-4/7 heterodimers in the vertebrate embryo.
影响因子：
10.5
作者：
HAWLEY, SHB;WUNNENBERGSTAPLETON, K;CHO, KWY
通讯作者：
CHO, KWY