Sensitive detection of copper(II) by a commercial glucometer using click chemistry
Sensitive detection of copper(II) by a commercial glucometer using click chemistry
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使用点击化学通过商用血糖仪灵敏检测铜 (II)
DOI：
10.1016/j.bios.2013.01.069
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发表时间：
2013-07-15
影响因子：
12.6
通讯作者：
Chai, Yaqin
中科院分区：
文献类型：
--
作者：
Su, Jiao;Xu, Jin;Chai, Yaqin
市售的血糖仪是迄今为止最成功的床旁（POC）传感器。然而，血糖仪只对葡萄糖而不是其他物质有反应。将血糖仪的使用扩展到监测不同类型的目标将可能彻底改变血糖仪的适用性。在这里，我们报告了一种新的传感策略，灵敏和选择性检测Cu 2+的基础上，多转化酶共轭磁珠信号放大标记和血糖传感器。Cu 2+被抗坏血酸钠原位还原为Cu+，抗坏血酸钠催化固定在一次性丝网印刷碳电极上的炔基-DNA与附着于转化酶/磁珠缀合物的叠氮基-DNA之间的点击连接。磁珠标记上的许多转化酶通过Cu+催化的点击化学反应将蔗糖转化为葡萄糖，葡萄糖由血糖仪监测，并为Cu 2+检测提供放大的数字读数。通过采用多转化酶信号放大，可以检测低至10 nM的Cu 2+。该方法对Cu ~（2+）具有很高的选择性，适用于真实的河流样品中Cu ~（2+）的检测。我们的策略可以很容易地扩展到监测范围广泛的目标时，再加上各种识别事件。(C)2013爱思唯尔有限公司版权所有。
The commercially available glucometer has been the most successful point-of-care (POC) sensor up to date. However, the glucometer only responds to glucose rather than other species. Extending the use of the glucometer for monitoring different types of targets would potentially revolutionize the applicability of the glucometer. Here we report a new sensing strategy for sensitive and selective detection of Cu2+ based on multi-invertase conjugated magnetic bead signal amplification labels and a glucometer transducer. The Cu2+ is in situ reduced to Cu+ by sodium ascorbate, which catalyzes the click linking between the alkynyl-DNA immobilized on a disposable screen printed carbon electrode and the azido-DNA attached to the invertase/magnetic bead conjugates. The numerous invertase on the magnetic bead labels through Cu+-catalyzed click chemistry reaction convert sucrose to glucose, which is monitored by the glucometer and offers amplified digital readings for Cu2+ detection. By employing the multi-invertase signal amplification, as low as 10 nM Cu2+ can be detected. Our method also shows high selectivity for Cu2+ against other metal ions owing to the highly specific Cu+-catalyzed click chemistry reaction, and is applicable for monitoring Cu2+ in real river samples. Our strategy can be easily expanded for the monitoring of a wide range of targets when coupled with various recognition events. (C) 2013 Elsevier B.V. All rights reserved.