DETERMINATION OF HYDROXYPROLINE IN TISSUE AND PROTEIN SAMPLES CONTAINING SMALL PROPORTIONS OF THIS IMINO ACID
DETERMINATION OF HYDROXYPROLINE IN TISSUE AND PROTEIN SAMPLES CONTAINING SMALL PROPORTIONS OF THIS IMINO ACID
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DOI：
10.1016/0003-9861(61)90291-0
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发表时间：
1961-01-01
影响因子：
3.9
通讯作者：
WOESSNER, JF
中科院分区：
文献类型：
--
作者：
WOESSNER, JF
提出了一种在4000份氨基酸中只含有1份羟脯氨酸的生物材料中羟脯氨酸的定量测定方法。该方法已应用于研究羟基脯氨酸在细胞颗粒、组织液和纯化的植物和动物蛋白中的分布。在胃蛋白酶、弹性蛋白酶和纤维蛋白的结晶制剂中发现了大量的羟脯氨酸。
A method is presented for the quantitative determination of hydroxyproline in biological materials containing as little as one part of hydroxyproline in 4000 parts of amino acids. This method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins. Significant amounts of hydroxyproline were found in crystalline preparations of pepsin, elastase, and ficin.