Accumulation of βm, a structural member of X,K-ATPase β-subunit family, in nuclear envelopes of perinatal myocytes
Accumulation of βm, a structural member of X,K-ATPase β-subunit family, in nuclear envelopes of perinatal myocytes
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DOI：
10.1152/ajpcell.00358.2003
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发表时间：
2004-04
影响因子：
5.5
通讯作者：
Hao Zhao;N. Pestov;T. Korneenko;M. Shakhparonov;N. Modyanov
中科院分区：
文献类型：
--
作者：
Hao Zhao;N. Pestov;T. Korneenko;M. Shakhparonov;N. Modyanov
最近发现的肌肉特异性βm蛋白在结构上与X，K-ATP酶β亚基密切相关。然而，它具有许多独特的性质，例如主要定位于细胞内储存，并且在异源共表达时与已知的X，K-ATP酶α亚基缺乏关联。本研究测定了小鼠βm基因的一级结构，并分析了该基因（ATP 1B 4）的发育调控。在妊娠第14天首次检测到表达，在第16天急剧增加，并在第18天达到最大值。出生后，表达迅速下降，在成年小鼠中几乎检测不到。进一步的亚细胞定位研究表明，βm不仅定位于肌浆网，而且在出生前后骨骼肌的核膜中都有分布。免疫组织化学研究表明，βm是特异性的心肌细胞，在亚细胞水平上，许多核膜被强烈标记，在两个...
Recently discovered muscle-specific βm protein is structurally closely related to the X,K-ATPase β-subunits. However, it has a number of unique properties such as predominant localization in intracellular stores and lack of association with known X,K-ATPase α-subunits on heterologous coexpression. In this study, the primary structure of mouse βm was determined and developmental regulation of the gene (ATP1B4) was analyzed. The expression is first detected at day 14 of gestation, is sharply increased at day 16, and reaches its maximum at day 18. After birth, the expression quickly decreases and is hardly detectable in adult mice. A more detailed subcellular localization study was undertaken, and its results indicate that βm not only is located in sarcoplasmic reticulum but is concentrated in nuclear envelopes of both prenatal and postnatal skeletal muscles. Immunohistochemical studies show that βm is specific to myocytes and, at the subcellular level, many nuclear envelopes are intensively labeled in both ...