Testicular development involves the spatiotemporal control of PDGFs and PDGF receptors gene expression and action.
Testicular development involves the spatiotemporal control of PDGFs and PDGF receptors gene expression and action.
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DOI：
10.1083/jcb.131.4.1105
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发表时间：
1995-11
期刊：
影响因子：
--
通讯作者：
Spera G
中科院分区：
文献类型：
--
作者：
Gnessi L;Emidi A;Jannini EA;Carosa E;Maroder M;Arizzi M;Ulisse S;Spera G
血小板源性生长因子（PDGF）是主要刺激间充质来源的细胞的趋化性、增殖和代谢的生长调节分子。在这项研究中，我们发现了高水平的PDGFs和PDGFs受体（PDGFRs）的mRNA，以及相应的蛋白质在大鼠睾丸中的特异性免疫染色。PDGFs和PDGFRs的表达显示出发育调节和组织特异性。PDGFs和PDGFRs基因的表达在出生前2d在整个睾丸RNA中观察到，出生后5天增加，并在成人中降至低水平。在出生前和出生后早期，表达PDGFs和PDGFRs基因转录本的主要细胞群分别是支持细胞和管周肌样细胞（PMC）或其前体，而在成年动物中，PDGFs和PDGFRs仅限于Leydig细胞。我们还发现，出生后早期的支持细胞产生PDGF样物质，这种生产是抑制剂量依赖性卵泡刺激素（FSH）。由PMC和支持细胞的PDGF的表达在时间顺序上对应于PMC增殖和从肾小管向管周迁移的发育期。此外，我们观察到，所有的PDGF亚型和早期出生后支持细胞的条件培养基显示出强烈的趋化活性PMC的抑制抗PDGF抗体。这些数据表明，通过PDGF配体和受体表达的时空模式，PDGF可能在睾丸发育和稳态中发挥作用。
Platelet-derived growth factors (PDGFs) are growth-regulatory molecules that stimulate chemotaxis, proliferation and metabolism primarily of cells of mesenchymal origin. In this study, we found high levels of PDGFs and PDGFs receptors (PDGFRs) mRNAs, and specific immunostaining for the corresponding proteins in the rat testis. PDGFs and PDGFRs expression was shown to be developmentally regulated and tissue specific. Expression of PDGFs and PDGFRs genes was observed in whole testis RNA 2 d before birth, increased through postnatal day 5 and fell to low levels in adult. The predominant cell population expressing transcripts of the PDGFs and PDGFRs genes during prenatal and early postnatal periods were Sertoli cells and peritubular myoid cells (PMC) or their precursors, respectively, while in adult animals PDGFs and PDGFRs were confined in Leydig cells. We also found that early postnatal Sertoli cells produce PDGF-like substances and that this production is inhibited dose dependently by follicle-stimulating hormone (FSH). The expression of PDGFRs by PMC and of PDGFs by Sertoli cells corresponds in temporal sequence to the developmental period of PMC proliferation and migration from the interstitium to the peritubulum. Moreover, we observed that all the PDGF isoforms and the medium conditioned by early postnatal Sertoli cells show a strong chemotactic activity for PMC which is inhibited by anti-PDGF antibodies. These data indicate that, through the spatiotemporal pattern of PDGF ligands and receptors expression, PDGF may play a role in testicular development and homeostasis.