Polymerase chain reaction for detection of herpes simplex virus (HSV) DNA on mucosal surfaces: Comparison with HSV isolation in cell culture
Polymerase chain reaction for detection of herpes simplex virus (HSV) DNA on mucosal surfaces: Comparison with HSV isolation in cell culture
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DOI：
10.1086/379043
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发表时间：
2003-11-01
影响因子：
6.4
通讯作者：
Corey, L
中科院分区：
文献类型：
--
作者：
Wald, A;Huang, ML;Corey, L
本研究通过实时定量聚合酶链反应（PCR）检测，比较了从296例单纯疱疹病毒（HSV）感染者的136，000份粘膜分泌物样本中分离HSV的比率与HSV DNA的检出率。总体而言，在3.0%的样本中分离出HSV，在12.1%的样本中检测到HSV DNA。HSV DNA拷贝数的平均数为10（4.9），在HSV病变存在的日子和10（4.4），从HSV病变不存在的日子的样品中获得的样品。在培养物中分离病毒的能力与样本中HSV DNA拷贝数的对数之间存在线性关系;这种关系持续存在于男性或女性样本、人类免疫缺陷病毒阴性或阳性参与者样本以及病变存在或不存在时获得的样本中。在家庭收集的标本中，PCR阳性与病毒培养阳性的比例从冬季的3.8：1上升到夏季的8.8：1，反映了在温暖天气下运输的病毒培养标本的不稳定性。
This study compared the rate of isolation of herpes simplex virus (HSV) from 136,000 samples of mucosal secretions obtained from 296 HSV-infected persons versus the rate of detection of HSV DNA, by means of a real-time quantitative polymerase chain reaction (PCR) assay. Overall, HSV was isolated in 3.0% of samples, and HSV DNA was detected in 12.1% of samples. The mean number of HSV DNA copies was 10(4.9) in samples obtained on days when HSV lesions were present and 10(4.4) in samples from days when HSV lesions were absent. There was a linear relationship between the ability to isolate virus in culture and the log number of copies of HSV DNA in the sample; this relationship persisted in samples from men or women, in samples from human immunodeficiency virus-negative or -positive participants, and in samples obtained on days when lesions were present or absent. In home-collected specimens, the ratio of PCR positivity to viral-culture positivity rose from 3.8:1 in the winter to 8.8:1 in the summer months, reflecting the lability of viral-culture specimens transported during warm weather.