TRANSLATIONAL BLOCKADE IMPOSED BY CYTOKINE-DERIVED UA-RICH SEQUENCES
TRANSLATIONAL BLOCKADE IMPOSED BY CYTOKINE-DERIVED UA-RICH SEQUENCES
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DOI：
10.1126/science.2672333
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发表时间：
1989-08-25
期刊：
影响因子：
56.9
通讯作者：
HUEZ, G
中科院分区：
文献类型：
--
作者：
KRUYS, V;MARINX, O;HUEZ, G
指定参与炎症反应的某些蛋白质和某些癌蛋白的信使rna在3‘’非翻译区含有一个保守的富含ua的序列。该序列由几个散布的辛核苷UUAUUUAU重复序列组成，已被证明在一些真核生物中破坏mRNA的稳定。然而，当mrna被转移到非洲爪蟾卵母细胞时，这种效应没有被观察到，这使得将稳定性与翻译调节分离开来成为可能。对于干扰素、粒细胞-巨噬细胞集落刺激因子和c-fos rna，观察到富含ua的序列可以阻止mRNA的翻译。
The messenger RNAs specifying certain proteins involved in the inflammatory response and certain oncoproteins contain a conserved UA-rich sequence in the 3'' untranslated region. This sequence, which is composed of several interspersed repeats of the octanucleotide UUAUUUAU, has been shown to destabilize mRNA in some eukaryotes. However, this effect is not seen when mRNAs are transferred to Xenopus oocytes, which made it possible to separate stability from translational regulation. For interferon, granulocyte-macrophage colony-stimulating factor, and c-fos RNAs, the UA-rich sequence was observed to preclude mRNA translation.