CHEMICAL AND STRUCTURAL-ANALYSIS OF THE RELATION BETWEEN CORTICAL INPUTS AND TYROSINE HYDROXYLASE-CONTAINING TERMINALS IN RAT NEOSTRIATUM
CHEMICAL AND STRUCTURAL-ANALYSIS OF THE RELATION BETWEEN CORTICAL INPUTS AND TYROSINE HYDROXYLASE-CONTAINING TERMINALS IN RAT NEOSTRIATUM
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DOI：
10.1016/0006-8993(84)90239-7
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发表时间：
1984-01-01
期刊：
影响因子：
2.9
通讯作者：
PICKEL, VM
中科院分区：
文献类型：
--
作者：
BOUYER, JJ;PARK, DH;PICKEL, VM
在成年大鼠单侧皮质剥脱后第2天和第12天的新纹状体中，检测了酪氨酸羟化酶（TH）水平以及大脑皮质轴突与TH阳性终末（主要是多巴胺能终末）之间的超微结构关系。从未受损和受损半球的尾状核进行TH的生化分析或处理的光或电子显微镜定位的酶。在这两个时间间隔检查，有没有统计学上显着的改变TH活性或明显的变化，通过光学显微镜观察到的反应性标记的强度。术后2天，对去皮质同侧的尾状核进行EM检查，发现存在许多小的嗜锇性终结，在对侧很少见到。进一步的超微结构检查表明，嗜锇终扣形成主要是不对称的，轴树突触。在包含退化和TH标记终端的部分中，可以发现2种连接模式。首先，也是最常见的，退化和TH标记的终端形成突触与同一树突或树突棘。不太常见的是，这两种类型的端子彼此直接接触。在这种轴-轴关系中，终末之间的外膜是并列的，但通常未能表现出突触前或突触后的专业化。皮质和多巴胺能黑质传出对大鼠尾状核中的共同受体神经元具有作用，并为这2种主要输入之间可能的直接轴突相互关系提供支持。
Levels of tyrosine hydroxylase (TH) and the ultrastructural relation between axons from cerebral cortex and TH containing, predominantly dopaminergic terminals were examined in the adult rat neostriatum at 2 and 12 days following unilateral decortication. The caudate nuclei from the unlesioned and lesioned hemispheres were biochemically assayed for TH or processed for light or electron microscopic localization of the enzyme. At both time intervals examined, there was no statistically significant alteration in TH activity or apparent change in the intensity of reactive labeling visualized by light microscopy. EM examination of the caudate nucleus homolateral to the decortication at 2 days following surgery revealed the presence of numerous small, osmiophilic boutons which were much less frequently seen on the contralateral side. Further ultrastructural examination showed that the osmiophilic boutons formed predominately asymmetric, axodendritic synapses. In sections containing both degenerating and TH labeled terminals, 2 patterns of connectivity could be discovered. First and most commonly, the degenerating and TH-labeled terminals formed synapses with the same dendrite or dendritic spine. Less frequently, the 2 types of terminals were in direct contact with each other. In this axo-axonic relation, the outer membranes between the terminals were in apposition but usually failed to exhibit pre- or postsynaptic specializations. The cortical and dopaminergic nigral efferents have actions on common recipient neurons in the rat caudate nucleus and provide support for a possible direct axonal interrelationship between these 2 primary inputs.