Controlled degradability of polysaccharide multilayer films in vitro and in vivo
Controlled degradability of polysaccharide multilayer films in vitro and in vivo
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DOI：
10.1002/adfm.200400588
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发表时间：
2005-11-01
影响因子：
19
通讯作者：
Voegel, JC
中科院分区：
文献类型：
--
作者：
Picart, C;Schneider, A;Voegel, JC
本文论证了多糖多层膜在体内和体外可降解性调控的可能性。壳聚糖和透明质酸多层膜（CHI/HA）采用不同浓度的水溶性碳二亚胺(1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDC）与n -羟基磺基琥珀酰亚胺结合进行天然或交联。用异硫氰酸荧光素（CHIFITC）标记壳聚糖后，用石英晶体微天平、荧光法和激光共聚焦扫描显微镜观察壳聚糖膜在溶菌酶和透明质酸酶接触下的体外降解情况。天然膜受到这些酶的降解，交联膜更耐酶降解。中等分子量的壳聚糖制备的膜比壳聚糖-低聚糖制备的膜具有更强的抗病性。薄膜也与等离子体接触，这引起了薄膜结构的变化，但对交联膜没有任何影响。体外实验表明，巨噬细胞能够降解各种类型的膜并内化壳聚糖。在小鼠腹腔内植入一周后，膜的体内降解再次显示原生膜几乎完全降解，而交联膜仅部分降解。综上所述，这些结果表明，多糖多层膜作为生物降解涂层具有潜在的体内应用价值，并且可以通过使用不同分子量的壳聚糖和控制膜交联来调节降解。
This article demonstrates the possibility of tuning the degradability of polysaccharide multilayer films in vitro and in vivo. Chitosan and hyaluronan multilayer films (CHI/HA) were either native or crosslinked using a water soluble carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) at various concentrations in combination with N-hydroxysulfosuccinimide. The in-vitro degradation of the films in contact with lysozyme and hyaluronidase was followed by quartz crystal microbalance measurements, fluorimetry, and confocal laser scanning microscopy after labeling of the chitosan with fluorescein isothiocyanate (CHIFITC). The native films were subjected to degradation by these enzymes, and the crosslinked films were more resistant to enzymatic degradation. Films made of chitosan of medium molecular weight were more resistant than films made of chitosan-oligosaccharides. The films were also brought in contact with plasma, which induced a change in film structure for the native film but did not have any effect on the crosslinked film. The in-vitro study shows that macrophages can degrade all types of films and internalize the chitosan. The in-vivo degradation of the films implanted in mouse peritoneal cavity for a week again showed an almost complete degradation of the native films, whereas the crosslinked films were only partially degraded. Taken together, these results suggest that polysaccharide multilayer films are of potential interest for in-vivo applications as biodegradable coatings, and that degradation can be tuned by using chitosan of different molecular weights and by controlling film crosslinking.