Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain.
Somatic mutation in genes for the variable portion of the immunoglobulin heavy chain.
复制标题
免疫球蛋白重链可变部分基因的体细胞突变。
DOI：
10.1126/science.6801765
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Capra,JD
中科院分区：
文献类型：
--
作者：
Sims,J;Rabbitts,TH;Estess,P;Slaughter,C;Tucker,PW;Capra,JD
检测了可能编码顶偶氮苯基阿索酸抗体的基因库的大小。利用杂交瘤的信使RNA构建了重链特异的全长互补DNA克隆，该杂交瘤产生抗arsate半抗原的抗体，并携带组成交叉反应独特型(CRI)的几乎全部决定因素。互补DNA克隆和相应的免疫球蛋白重链的序列已经被独立测定。从原始的重链互补DNA克隆中制备可变区基因的探针，用Southern Filter杂交方法分析A/J(CRI阳性)和BALB/c(CRI阴性)小鼠的基因组DNA。在两个菌株中都检测到了大约20到25个含有“生殖系”可变区基因片段的限制性内切酶片段，其中许多片段是两个菌株所共有的，因为到目前为止已经对35个CRI阳性重链进行了部分测序，其中31个是不同的，杂交分析的结果表明，涉及重链可变区基因片段的体细胞突变事件在该系统中氨基酸序列多样性的起源中起到了作用。
The size of the gene pool potentially encoding antibodies top-azophenyl arsonate has been examined. A heavy chain-specific full-length complementary DNA clone has been constructed with the use of messenger RNA from a hybridoma that produces antibodies to the arsonate hapten and bears nearly a full complement of the determinants comprising the cross-reactive idiotype (CRI). The sequences of both the complementary DNA clone and the corresponding immunoglobulin heavy chain have been independently determined. A probe for the variable region gene was prepared from the original heavy chain complementary DNA clone and used to analyze, by Southern filter hybridization, genomic DNA from both A/J (CRI positive) and BALB/c (CRI negative) mice. Approximately 20 to 25 restriction fragments containing "germline" variable region gene segments were detected in both strains, and many are shared by both, Since 35 CRI-positive heavy chains have been partially sequenced thus far and 31 are different, the results of the hybridization analysis suggest that somatic mutation events involving the variable region gene segments of the heavy chain play a role in the origin of the amino acid sequence diversity seen in this system.