CYCLIC ELECTROCHEMICAL INACTIVATION AND RESTORATION OF COMPETENCE OF BONE MATRIX TO TRANSFORM FIBROBLASTS
CYCLIC ELECTROCHEMICAL INACTIVATION AND RESTORATION OF COMPETENCE OF BONE MATRIX TO TRANSFORM FIBROBLASTS
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DOI：
10.1073/pnas.71.5.1648
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
HUGGINS, CB
中科院分区：
文献类型：
--
作者：
REDDI, AH;HUGGINS, CB
大鼠骨基质短暂暴露于高电荷电化学物质中，对其将成纤维细胞转化为软骨母细胞和成骨细胞的能力有深刻但可逆的影响。这一功能的抑制和随后的重新激活受到电化学试剂的电荷和反应混合物的pH的严重影响。在稀酸中，长电负性分子伊文思蓝抑制转化能力，而聚阳离子季铵盐碱六甲撑不能抑制转化能力。伊文思蓝处理骨基质，然后用己二胺处理骨基质，实现了失活-转化能力恢复的循环；组织化学证实了一种电化学复合体。在稀碱中，己二甲基抑制骨基质的转化功能，而伊文思蓝不抑制骨基质的转化功能。骨基质表面的电荷特性对成纤维细胞的转化属性具有重要意义。
Brief exposure of rat bone matrix to highly charged electrochemicals had profound but reversible effects on its competence to transform fibroblasts into chondroblasts and osteoblasts. Suppression and subsequent reactivation of this function were influenced critically by the charge of the electrochemical reagent and the pH of the reaction mixture. In dilute acids, Evans Blue, a long electronegative molecule, suppressed transforming competence, whereas hexadimethrine, a polycationic quaternary ammonium base, failed to do so. A cycle of inactivation-restoration of transforming competence was achieved by sequential treatment of bone matrix with Evans Blue followed by hexadimethrine; an electrochemical complex was demonstrated by histochemistry. In dilute alkalies, hexadimethrine inhibited, whereas, Evans Blue did not suppress the transforming function of the bone matrix. Electric charge characteristics on the surface of the bone matrix are of crucial significance for the fibroblast-transforming attribute.