PAX-2 IS A DNA-BINDING PROTEIN EXPRESSED IN EMBRYONIC KIDNEY AND WILMS-TUMOR
PAX-2 IS A DNA-BINDING PROTEIN EXPRESSED IN EMBRYONIC KIDNEY AND WILMS-TUMOR
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DOI：
10.1073/pnas.89.4.1179
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发表时间：
1992-02-15
影响因子：
11.1
通讯作者：
DOUGLASS, EC
中科院分区：
文献类型：
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作者：
DRESSLER, GR;DOUGLASS, EC
小鼠Pax-2基因包含一个与果蝇配对盒同源的蛋白质编码域，首次在某些节段型发育控制基因中被描述。多克隆抗体识别两种由差异剪接的mRNAs编码的Pax-2蛋白。Pax-2蛋白可以结合已知与果蝇蛋白的配对结构域相互作用的DNA序列。免疫细胞化学显示，在发育过程中，Pax-2的表达定位于致密间充质细胞及其上皮衍生物的胞核。随着肾小管上皮细胞的分化，其表达水平突然下调。在人肾母细胞瘤的上皮细胞中也可检测到高水平的Pax-2表达。这些数据表明，在肾发育早期和肾母细胞瘤中，Pax-2是一种活跃的转录因子，存在于间充质向上皮间充质转化过程中。
The murine Pax-2 gene contains a protein coding domain homologous to the Drosophila paired-box, first described in certain developmental control genes of the segmentation type. Polyclonal antibodies recognize two Pax-2 Proteins that are encoded by differentially spliced mRNAs. The Pax-2 proteins can bind a DNA sequence known to interact with the paired domain of a Drosophila protein. By immunocytochemistry, expression of Pax-2 could be localized to the nuclei of condensing mesenchyme cells and their epithelial derivatives in the developing kidney. Expression is abruptly down-regulated as the tubular epithelium differentiates. High levels of Pax-2 expression could also be detected in the epithelial cells of human Wilms tumors. These data suggest that Pax-2 is a transcription factor active during the mesenchyme-to-epithelium transition in early kidney development and in Wilms tumor.