Nitrogen Gas Fixation and Conversion to Ammonium Using Microbial Electrolysis Cells
Nitrogen Gas Fixation and Conversion to Ammonium Using Microbial Electrolysis Cells
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DOI：
10.1021/acssuschemeng.8b05763
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发表时间：
2019
影响因子：
8.4
通讯作者：
Juan F. Ortiz-Medina;A. Grunden;M. Hyman;D. Call
中科院分区：
文献类型：
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作者：
Juan F. Ortiz-Medina;A. Grunden;M. Hyman;D. Call
氨 (NH3) 是一种重要的工业化学品，采用能源和碳密集型哈伯-博世工艺生产。从固定氮气 (N2) 的微生物中回收 NH3 可能会提供一种可持续的替代方案，因为它们的专门固氮酶可以将 N2 还原为铵 (NH4+)，而无需高温高压。本研究探讨了使用厌氧单室微生物电解池 (MEC) 将 N2 转化为 NH4+ 的可能性。当阳极和阴极之间施加的电压从 0.7 V 增加到 1.0 V 并达到最大 ~40 nmol C2H4 min–1 mg Protein–1 时，微生物群落的 N2 固定率 [基于乙炔气 (C2H2) 到乙烯气 (C2H4) 转化测定] 显着增加，这与模型需氧 N2 固定细菌相当。 NH4+的存在可以抑制固氮酶的活性，但不会显着降低N2固定率。添加蛋氨酸亚砜亚胺后，NH4+ 的吸收...
Ammonia (NH3) is an important industrial chemical that is produced using the energy- and carbon-intensive Haber-Bosch process. Recovering NH3 from microorganisms that fix nitrogen gas (N2) may provide a sustainable alternative because their specialized nitrogenase enzymes can reduce N2 to ammonium (NH4+) without the need for high temperature and pressure. This study explored the possibility of converting N2 into NH4+ using anaerobic, single-chamber microbial electrolysis cells (MECs). N2 fixation rates [based on an acetylene gas (C2H2) to ethylene gas (C2H4) conversion assay] of a microbial consortium increased significantly when the applied voltage between the anode and cathode increased from 0.7 to 1.0 V and reached a maximum of ∼40 nmol of C2H4 min–1 mg protein–1, which is comparable to model aerobic N2-fixing bacteria. The presence of NH4+, which can inhibit the activity of the nitrogenase enzyme, did not significantly reduce N2 fixation rates. Upon addition of methionine sulfoximine, an NH4+ uptake i...