Separation and quantitation of polyethylene glycols 400 and 3350 from human urine by high-performance liquid chromatography.
Separation and quantitation of polyethylene glycols 400 and 3350 from human urine by high-performance liquid chromatography.
复制标题
DOI：
10.1002/jps.2600810412
复制
发表时间：
1992-04
影响因子：
3.8
通讯作者：
Colleen M. Ryan;M. Yarmush;R. Tompkins
中科院分区：
文献类型：
--
作者：
Colleen M. Ryan;M. Yarmush;R. Tompkins
聚乙二醇3350（PEG 3350）可用作口服给药探针以测量体内肠对大分子的渗透性。先前检测尿液中排泄的聚乙二醇（PEG）的方法受到与液-液萃取和浊度分析相关的固有不准确性的阻碍。为了通过先前的方法进行准确定量，需要放射性标记。本文描述了一种分离和定量人尿液中PEG 3350和PEG 400的方法，该方法不依赖于放射性标记，在临床实践中是准确的。该方法使用上胶的再生纤维素膜和混合离子交换树脂进行样品制备，并使用具有折射率检测的高效液相色谱进行分析。正常个体口服40 g PEG 3350和5 g PEG 400后的24小时排泄量分别为PEG 3350原始剂量的0.12 +/- 0.04%和PEG 400原始剂量的26.3 +/- 5.1%。
Polyethylene glycol 3350 (PEG 3350) is useful as an orally administered probe to measure in vivo intestinal permeability to macromolecules. Previous methods to detect polyethylene glycol (PEG) excreted in the urine have been hampered by inherent inaccuracies associated with liquid-liquid extraction and turbidimetric analysis. For accurate quantitation by previous methods, radioactive labels were required. This paper describes a method to separate and quantitate PEG 3350 and PEG 400 in human urine that is independent of radioactive labels and is accurate in clinical practice. The method uses sized regenerated cellulose membranes and mixed ion-exchange resin for sample preparation and high-performance liquid chromatography with refractive index detection for analysis. The 24-h excretion for normal individuals after an oral dose of 40 g of PEG 3350 and 5 g of PEG 400 was 0.12 +/- 0.04% of the original dose of PEG 3350 and 26.3 +/- 5.1% of the original dose of PEG 400.