A comparative study of virus isolation, polymerase chain reaction, and antigen detection in children of mothers infected with human immunodeficiency virus.
A comparative study of virus isolation, polymerase chain reaction, and antigen detection in children of mothers infected with human immunodeficiency virus.
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人类免疫缺陷病毒母亲感染儿童的病毒分离、聚合酶链反应和抗原检测的比较研究。
DOI：
10.1016/s0022-3476(05)82823-9
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
McIntosh,K
中科院分区：
文献类型：
--
作者：
Krivine,A;Yakudima,A;LeMay,M;Pena-Cruz,V;Huang,AS;McIntosh,K
我们报告了一项旨在比较聚合酶链反应（PCR）与培养和p24测定在婴儿和儿童中诊断人类免疫缺陷病毒（HIV）感染的调查。研究人员对45名携带1型艾滋病毒抗体的母亲所生的孩子进行了研究；检测血浆中P24抗原，PCR扩增后在外周血单核细胞中寻找HIV-1前病毒DNA。26例血液标本进行HIV培养；除了两种情况外，所有的培养都是在进行PCR检测的同时建立的。采用原病毒基因组三个区域的引物对进行PCR检测。PCR检测结果与培养结果吻合良好；在同时进行两项检测的24例患儿中，10例培养和PCR检测均为阳性，1例PCR检测阳性但培养阴性，13例两项检测均为阴性（一致性96%）。相比之下，血浆中p24抗原的测定是一种不敏感的感染标志物：12名培养阳性的婴儿中有6名p24检测结果阳性，18名婴儿中有8名PCR检测结果阳性。16名随后HIV-1血清阴性的儿童PCR结果为阴性。本研究提供了进一步的证据，证明PCR检测是一种有效的替代病毒培养诊断儿童HIV感染。
We report on an investigation designed to compare the polymerase chain reaction (PCR) with culture and p24 measurement for the diagnosis of human immunodeficiency virus (HIV) infection in infants and children. Forty-five children born of mothers with antibodies to HIV type 1 were studied; P24 antigen was measured in plasma, and HIV-1 proviral DNA was sought in peripheral blood mononuclear cells after amplification by PCR. In 26 cases, blood specimens were cultured for HIV; in all but two instances cultures were established at the same time that the PCR test was performed. Primer pairs in three regions of the proviral genome were used for the PCR test. There was good agreement between the results obtained from PCR tests and from cultures; of 24 children in whom both tests were done at the same time, 10 had positive results on both the culture and the PCR test, 1 had positive results on the PCR test but negative culture results, and 13 had negative results on both tests (concordance 96%). Measurement of p24 antigen in plasma was, in contrast, an insensitive marker of infection: 6 of 12 infants with positive cultures had positive p24 test results, and 8 of 18 infants had positive PCR test results. Sixteen children with subsequent seronegativity for HIV-1 had negative PCR results. This study provides further evidence that the PCR test is a valid alternative to viral culture for the diagnosis of pediatric HIV infection.
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人类免疫缺陷病毒1型快速灵敏的病毒培养方法
影响因子：
9.4
作者：
J. Jackson;Robert W. Coombs;K. Sannerud;F. Rhame;H H Balfour
通讯作者：
H H Balfour
围产期人类免疫缺陷病毒感染。
影响因子：
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作者：
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R. Wedgwood
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DOI：
--
发表时间：
1987
期刊：
The Lancet
影响因子：
--
作者：
W. Borkowsky;Deborah Paul;D. Bebenroth;K. Krasinski;T. Moore;S. Chandwani
通讯作者：
S. Chandwani
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影响因子：
3.3
作者：
W. Borkowsky;K. Krasinski;D. Paul;R. Holzman;T. Moore;D. Bebenroth;R. Lawrence;S. Chandwani
通讯作者：
S. Chandwani
对 Wilink 报告的第二个想法。
DOI：
--
发表时间：
1960
期刊：
The Lancet
影响因子：
--
作者：
F. Lafitte;J. Squire
通讯作者：
J. Squire