Effect of genistein on the gene and protein expressions of CXCL-12 and EGR-1 in the rat ovary.
Effect of genistein on the gene and protein expressions of CXCL-12 and EGR-1 in the rat ovary.
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DOI：
10.1111/jpn.13448
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发表时间：
2020-09
影响因子：
2.7
通讯作者：
Tao Zhang;X. Chi;Fan-Xiu Kong;X. Chu
中科院分区：
文献类型：
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作者：
Tao Zhang;X. Chi;Fan-Xiu Kong;X. Chu
研究了染料木黄酮（GEN）对年轻和初老（衰老过程早期阶段）雌性大鼠卵巢组织中基质细胞衍生因子 -1/CXCL -12和早期生长反应基因 -1基因表达水平的影响。选取40只2 - 3月龄（200 ± 20 g）的年轻雌性斯普拉格 - 道利（SD）大鼠和40只10 - 12月龄（490 ± 20 g）的初老雌性SD大鼠。根据体重将大鼠分为对照组、低剂量组（L）、中剂量组（M）和高剂量组（H），分别给予15、30和60 mg/kg的GEN。阳性对照组（雌激素组）给予0.5 mg/kg己烯雌酚。处理持续30天。通过实时荧光定量PCR检测C - X - C趋化因子配体12（CXCL -12）和早期生长反应因子 -1（EGR -1）的mRNA表达，通过蛋白质印迹法检测EGR -1的蛋白表达。与阴性对照组（NC）相比，年轻大鼠GEN组的卵巢/体重比值下降，但差异不显著。同样，与NC相比，初老大鼠的卵巢/体重比值也随着GEN浓度的增加而下降，且在M组和H组下降显著（p <.01）。GEN的使用提高了卵巢中CXCL -12和EGR -1的基因及蛋白表达水平。皮尔逊相关性分析表明CXCL -12和EGR -1之间存在协同作用。因此，我们得出结论，GEN对初老组中CXCL -12和EGR -1的作用比年轻组更明显。
The effect of genistein (GEN) on the gene expression level of stromal cell-derived factor-1/CXCL-12 and early growth response gene-1 was studied in ovarian tissue of young and initially ageing (early stages in the ageing process) female rats. Forty, young female Sprague Dawley (SD) rats of 2-3 months old (200 ±20 g) and forty, initially ageing female SD rats of 10-12 months (490 ± 20 g) old were selected. According to the weight, rats were divided into control group, low-dose group (L), medium-dose group (M) and a high-dose group (H) and were given 15, 30 and 60 mg/kg GEN respectively. The positive control (Oestrogen) group was given 0.5 mg/kg diethylstilbestrol. The treatment lasted for 30 days. The mRNA expression of C-X-C motif chemokine ligand 12 (CXCL-12) and early growth response factor-1 (EGR-1) was measured by real-time PCR, and protein expression of EGR-1 was detected by Western blot. When compared to the negative control group (NC), the ovary/body weight ratio in the young rats decreased in the GEN group, but the difference was not significant. Similarly, compared with NC, the ovary/body weight ratio in the initially ageing rats also decreased with the increase in GEN concentration, but the decrease was significant in M and H groups (p < .01). The administration of GEN enhanced both the gene and protein expression levels of CXCL-12 and EGR-1 in the ovary. Pearson's correlation analysis showed a synergistic effect between CXCL-12 and EGR-1. Thus, we conclude that the effect of GEN on CXCL-12 and EGR-1 in the initially ageing group was obvious than that in the younger group.