CYCLIC-AMP INHIBITION OF FMET-LEU-PHE-DEPENDENT METABOLIC RESPONSES IN HUMAN-NEUTROPHILS IS NOT DUE TO ITS EFFECTS ON CYTOSOLIC CA-2+
CYCLIC-AMP INHIBITION OF FMET-LEU-PHE-DEPENDENT METABOLIC RESPONSES IN HUMAN-NEUTROPHILS IS NOT DUE TO ITS EFFECTS ON CYTOSOLIC CA-2+
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DOI：
10.1042/bj2240629
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发表时间：
1984-01-01
影响因子：
4.1
通讯作者：
DIVIRGILIO, F
中科院分区：
文献类型：
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作者：
DETOGNI, P;CABRINI, G;DIVIRGILIO, F
环磷酸腺苷能有效地抑制依赖fMet-Leu-Phe的呼吸爆发和嗜天青颗粒和特定颗粒的胞吐作用，而不影响细胞内钙离子的释放。FMet-Leu-Phe诱导的[Ca~(2+)]i升高在环状AMP处理的细胞中是短暂的，与未处理的细胞在无外界Ca~(2+)刺激下的升高相似。然而，在这些细胞中，fMet-Leu-Phe诱导代谢激活。因此，我们认为cAMP对中性粒细胞反应的抑制作用不是由于它对[Ca~(2+)]i稳态的影响。
Cyclic AMP powerfully inhibits the fMet-Leu-Phe-dependent respiratory burst and exocytosis of azurophilic and specific granules without affecting Ca2+ release from intracellular stores. The elevation of [Ca2+]i induced by fMet-Leu-Phe is short-lived in cyclic AMP-treated cells and similar to that of untreated cells stimulated in the absence of external Ca2+. Nevertheless, in these latter cells fMet-Leu-Phe induces metabolic activation. We therefore suggest that the inhibitory action of cyclic AMP on neutrophil responses is not due to its effects on [Ca2+]i homoeostasis.