Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats
Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats
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探讨PITX1基因新变异及其对奶山羊产奶性能的影响
DOI：
10.1016/s2095-3119(13)60212-9
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发表时间：
2013-01-01
影响因子：
4.8
通讯作者：
Chen Hong
中科院分区：
文献类型：
--
作者：
Lan Xian-yong;Zhao Hai-yu;Chen Hong
成对样同源域转录因子1（PITX1）通过间接调控生长激素（GH）和催乳素（PRL）基因的表达，在垂体发育中发挥重要作用，因此PITX1基因被视为构建基因多态性与乳性状关系的潜在候选基因。本研究旨在探寻奶山羊PITX1基因的新型遗传变异及其对产奶性能的影响。在此，我们报道了在P1位点山羊PITX1基因第一内含子第41位核苷酸处存在一种新型遗传变异（NW_00314033：g.201G>A或IVS1+41G>A），可通过Msp I PCR - RFLP技术进行基因分型。在Msp I PCR - RFLP分析中，关中奶山羊群体中GG变异为主要基因型，A变异为次要等位基因，且该群体处于哈迪 - 温伯格不平衡状态（卡方值χ² = 140，P （此处原文P后似乎缺少具体数值） ）
Paired-like homeodomain transcription factor 1 (PITX1) plays an important role in pituitary development by indirectly regulating the expression of the GH and PRL genes, and therefore PITX1 gene is regarded as a potential candidate gene for building the relationship between the gene polymorphism and milk traits. The aim of this study was to explore the novel genetic variant in PITX1 gene and its effect on milk performance in dairy goats. Herein, a novel genetic variation (NW_00314033: g.201G>A or IVS1+41G>A) located at nt41 position of the first intron of the goat PITX1 gene was reported at the P1 locus, which can be genotyped by the Msp I PCR-RFLP. In the Msp I PCR-RFLP analyis, the GG variant was a major genotype, and the A variant was a minor allele in Guanzhong dairy goats which was at Hardy-Weinberg disequilibrium (chi-square chi(2)=140, P