Analysis of the T cell receptor variability of tumor-infiltrating lymphocytes in colorectal carcinomas
Analysis of the T cell receptor variability of tumor-infiltrating lymphocytes in colorectal carcinomas
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DOI：
10.1159/000030008
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发表时间：
1998-05-01
期刊：
影响因子：
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通讯作者：
Farthmann, EH
中科院分区：
文献类型：
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作者：
Baier, PK;Wimmenauer, S;Farthmann, EH
虽然在某些肿瘤（如恶性黑色素瘤）中有良好的临床和实验证据，但结直肠癌（CRC）的免疫原性仍未确定。我们检查了4例结直肠癌患者的手术标本中肿瘤浸润淋巴细胞（TIL）中的T细胞克隆。肿瘤中特定淋巴细胞克隆的生长表明了体内的免疫反应。我们使用T细胞受体V β家族特异性半定量PCR和额外的测序来检测TIL克隆扩增。在结直肠癌中，特异性T细胞克隆未被证实。然而，我们在4个肿瘤中的3个中观察到V β 9的优势。
While there is good clinical and experimental evidence for immunological tumor control in some tumors-malignant melanomas, for instance-the immunogenicity of colorectal carcinoma (CRC) still remains unsettled. We examined surgical specimens from 4 CRC patients for T cell clones among tumor-infiltrating lymphocytes (TIL). The growth of specific lymphocyte clones in a tumor indicates an immunological response in vivo. We used a T cell receptor V beta family-specific semiquantitative PCR with additional sequencing to examine TIL for clonal expansion. In CRC, Specific T cell clones could not be demonstrated. However, we observed a predominance of V beta 9 in 3 of 4 tumors.