Plasma total homocysteine quantification: an improvement of the classical high-performance liquid chromatographic method with fluorescence detection of the thiol-SBD derivatives
Plasma total homocysteine quantification: an improvement of the classical high-performance liquid chromatographic method with fluorescence detection of the thiol-SBD derivatives
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DOI：
10.1016/s1570-0232(02)00401-4
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发表时间：
2002-11-05
影响因子：
3
通讯作者：
Apitz-Castro, R
中科院分区：
文献类型：
--
作者：
Garcia, AJ;Apitz-Castro, R
建立了一种快速、简便、等度高效液相色谱法测定血浆总同型半胱氨酸的方法。在该方法中，对样品制备进行了修改，以将硫醇还原的时间从室温下的30分钟减少到在37 ℃下用三-（2-羧乙基）膦（TCEP）还原的10分钟，将衍生化的时间从60分钟减少到在60 ℃下的10分钟，并通过可商购的较短HPLC柱洗脱SBD-硫醇衍生物。SBD-同型半胱氨酸衍生物在3.7分钟洗脱。该方法与其他先前描述的方法一样精确且更快地定量血浆中的tHcy，并且对于必须分析大量样本的流行病学研究非常有用（C）2002 Elsevier Science B. V.保留所有权利。
A rapid, shorter, isocratic high-performance liquid chromatographic method is described for the determination of plasma total homocysteine. In this method the sample preparation was modified for reduction of the time of the thiolic reduction from 30 at room temperature to 10 min at 37degreesC with tris-(2-carboxyethyl) phosphine (TCEP), reduction the time of derivatization from 60 to 10 ruin at 60degreesC and elution of the SBD-thiols derivative by a shorter HPLC-column which is commercially available. The SBD-homocysteine derivative was eluted at 3.7 min. The method was equally precise and faster for quantification of tHcy in plasma as other previously described method and should be very useful for epidemiological studies in which large numbers of samples have to be analyzed (C) 2002 Elsevier Science B.V. All rights reserved.