Anti-inflammatory efficacy of Licochalcone A:: correlation of clinical potency and in vitro effects
Anti-inflammatory efficacy of Licochalcone A:: correlation of clinical potency and in vitro effects
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DOI：
10.1007/s00403-006-0654-4
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发表时间：
2006-06-01
影响因子：
3
通讯作者：
Wenck, Horst
中科院分区：
文献类型：
--
作者：
Kolbe, Ludger;Immeyer, Jeannine;Wenck, Horst
甘草查耳酮 A (LicA) 是甘草属膨胀甘草的主要酚类成分，最近有报道称其具有抗炎和抗微生物作用。这些抗炎特性可用于 LicA 的局部应用。我们进行了前瞻性随机对照临床试验，以评估含有 LicA 的化妆品配方在剃须后皮肤刺激模型和紫外线引起的红斑形成中的抗刺激功效。临床试验还伴随着一系列体外实验，以表征 LicA 对几种皮肤病学相关细胞类型的抗炎特性。在剃须和紫外线引起的红斑测试中，与媒介物对照相比，外用 LicA 导致红斑显着减少，这证明了 LicA 的抗刺激特性。此外，LicA 是体外促炎反应的有效抑制剂，包括 N-甲酰基-MET-LEU-PHE (fMLP) 或酵母聚糖诱导的粒细胞氧化爆发、UVB 诱导的角质形成细胞释放 PGE(2)、脂多糖 (LPS) 诱导的成体真皮成纤维细胞释放 PGE(2)、fMLP 诱导的粒细胞释放 LTB4 以及 LPS 诱导的 PGE(2) 释放。单核细胞衍生的树突状细胞分泌 IL-6/TNF-α。报告的数据表明，当应用于敏感或受刺激的皮肤时，LicA 具有治疗性皮肤护理功效。
Licochalcone A (LicA), a major phenolic constituent of the licorice species Glycyrrhiza inflata, has recently been reported to have anti-inflammatory as well as anti-microbial effects. These anti-inflammatory properties might be exploited for topical applications of LicA. We conducted prospective randomized vehicle-controlled clinical trials to assess the anti-irritative efficacy of cosmetic formulations containing LicA in a post-shaving skin irritation model and on UV-induced erythema formation. The clinical trials were accompanied by a series of in vitro experiments to characterize anti-inflammatory properties of LicA on several dermatologically relevant cell types. Topical LicA causes a highly significant reduction in erythema relative to the vehicle control in both the shave- and UV-induced erythema tests, demonstrating the anti-irritative properties of LicA. Furthermore, LicA is a potent inhibitor of pro-inflammatory in vitro responses, including N-formyl-MET-LEU-PHE (fMLP)- or zymosan-induced oxidative burst of granulocytes, UVB-induced PGE(2) release by keratinocytes, lipopolysaccharide (LPS)-induced PGE(2) release by adult dermal fibroblasts, fMLP-induced LTB4 release by granulocytes, and LPS-induced IL-6/TNF-alpha secretion by monocyte-derived dendritic cells. The reported data suggest therapeutic skin care benefits from LicA when applied to sensitive or irritated skin.