Vacuolar lesion profile in sheep scrapie:: Factors influencing its variation and relationship to disease-specific PrP accumulation
Vacuolar lesion profile in sheep scrapie:: Factors influencing its variation and relationship to disease-specific PrP accumulation
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DOI：
10.1053/jcpa.2002.0558
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发表时间：
2002-07-01
影响因子：
0.8
通讯作者：
Jeffrey, M
中科院分区：
文献类型：
--
作者：
Begara-McGorum, I;González, L;Jeffrey, M
对 42 只具有与痒病症状相符的临床症状的羊的大脑进行了详细的空泡病变神经病理学检查。根据羊的品种（无角多塞特羊、切维厄特羊、威尔士山羊、设得兰羊和萨福克羊）、密码子 136、154 和 171 处的 PrP 基因型（VRQ/ VRQ、VRQ/ARQ、VRQ/ARR 和 ARQ/ARQ）以及感染类型（SSBP/1 实验感染或自然疾病）进行分组。检查来自 7 个脑区的 22 个神经解剖部位的神经毡中的空泡，并检查 obex 水平的 5 个部位的神经元内空泡。在一项早期研究中，还对 36 只绵羊的相同大脑区域进行了疾病特异性 PrP (Prp(d)) 积累的免疫组织化学检查。总神经纤维空泡化的程度在自然感染的 ARQ/ARQ 萨福克羊中最高，而在实验感染的 VRQ/VRQ Cheviot 羊和 VRQ/ARR Poll-Dorset 羊中最低。所检查的 22 个神经解剖部位中的 9 个部位的神经纤维空泡形成的严重程度被用来生成空泡病变概况，该概况显示了不同绵羊组之间的差异。这些变异可能归因于 PrP 基因型和绵羊品种，也可能归因于痒病剂；然而，羊群内的病变特征存在相当大的个体差异。所有组在 obex 水平上都表现出相似的神经纤维空泡与神经元空泡的比率。尽管通常观察到神经纤维网空泡化与 Prp(d) 沉积之间呈正相关，但除了与星形胶质细胞相关的 Prp(d) 积累模式外，该相关性较低。该研究表明，绵羊的空泡病变特征受到多种因素的影响，并且与小鼠的病变特征相比，对于区分瘙痒病菌株来说，其价值非常有限。 (C) 2002 年，爱思唯尔科学有限公司出版。
Detailed neuropathological examination for vacuolar lesions was performed on the brains of 42 sheep with clinical signs compatible with scrapie. The sheep were grouped according to their breed (Poll-Dorset, Cheviot, Welsh Mountain, Shetland and Suffolk), their PrP genotype at codons 136, 154 and 171 (VRQ/ VRQ, VRQ/ARQ, VRQ/ARR and ARQ/ARQ) and the type of infection (experimental infection with SSBP/1, or natural disease). Twenty-two neuroanatomical sites from seven brain regions were examined for vacuolation in the neuropil and five sites at the level of the obex were examined for intraneuronal vacuolation. In 36 sheep, immunohistochemical examination for disease-specific PrP (Prp(d)) accumulation had also been performed in the same brain regions in an earlier study. The magnitude of total neuropil vacuolation was highest in the naturally affected ARQ/ARQ Suffolk sheep and lowest in the experimentally infected VRQ/VRQ Cheviot sheep and VRQ/ARR Poll-Dorset sheep. The severity of neuropil vacuolation at nine of the 22 neuroanatomical sites examined was used to generate a vacuolar lesion profile, which showed variations between the different sheep groups. These variations could be attributed to both PrP genotype and sheep breed and also possibly to scrapie agent; there was, however, considerable individual variation in lesion profile within sheep groups. All groups showed a similar ratio of neuropil vacuolation to neuronal vacuolation at the level of the obex. Although a positive correlation between neuropil vacuolation and Prp(d) deposition was generally observed, it was low except for the astrocyte-associated pattern of Prp(d) accumulation. The study suggests that vacuolar lesion profiles in sheep are affected by several factors and, by comparison with lesion profiles in mice, are of no more than limited value for discriminating between scrapie strains. (C) 2002 Published by Elsevier Science Ltd.