Natural Infection of Lutzomyia neivai and Lutzomyia sallesi (Diptera: Psychodidae) by Leishmania infantum chagasi in Brazil
Natural Infection of Lutzomyia neivai and Lutzomyia sallesi (Diptera: Psychodidae) by Leishmania infantum chagasi in Brazil
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DOI：
10.1603/033.046.0525
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发表时间：
2009-09-01
影响因子：
2.1
通讯作者：
Andrade Filho, Jose D.
中科院分区：
文献类型：
--
作者：
Saraiva, Lara;Carvalho, Gustavo M. L.;Andrade Filho, Jose D.
在巴西米纳斯吉拉斯州的拉桑斯（Lassance）前，在雌性白蛉沙蝇Lutzomyia neivai（平托）（=Nyssomyia neivai）和Lutzomyia sallesi（Galvao & Coutinho）（=Evandromyia sallesi）（双翅目：蝇科）中发现了利什曼原虫的自然感染。前鞭毛体被发现在前物种的幽门区和后物种的腹部中肠。将通过显微镜检查发现感染的昆虫在盐水溶液中浸渍并接种到仓鼠中。随后的聚合酶链反应-限制性片段长度多态性分析显示，这两个菌株属于婴儿利什曼原虫，查加斯库尼亚和查加斯种。
Natural infections with Leishmania were found in females of the phlebotomine sand flies Lutzomyia neivai (Pinto) (=Nyssomyia neivai) and Lutzomyia sallesi (Galvao & Coutinho) (=Evandromyia sallesi) (Diptera: Psychodidae) front Lassance, in the Brazilian state of Minas Gerais. Promastigotes were found in the pyloric region of the former species and in the abdominal midgut of the latter species. Insects found to be infected by microscopic examination were macerated in saline solution and inoculated into hamsters. Subsequent analysis by polymerase chain reaction-restriction fragment length polymorphism revealed both isolates to belong to the species Leishmania infantum, chagasi Cunha & Chagas.